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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||10 Antibodies||22 Antibodies|
|Immunochromatography (IC)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||11 Antibodies||14 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||16 Antibodies||19 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||9 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||2 Antibodies|
|Western Blotting (WB)||24 Antibodies||34 Antibodies|
|Antigen||WAS Protein Family, Member 1 (WASF1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This WASF1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-WASF1 AntibodyTarget Details WASF1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of WAVE1 protein. (region surrounding Ile119)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details WASF1Product Details anti-WASF1 Antibody Application Details Handling Images back to top
|Alternative Name||WASF1 / WAVE1 (WASF1 Antibody Abstract)|
|Background||WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.Synonyms: KIAA0269, SCAR1, Verprolin homology domain-containing protein 1, WASP family protein member 1, WAVE-1, Wiskott-Aldrich syndrome protein family member 1|
|Molecular Weight||approx. 65 kDa|
Application DetailsProduct Details anti-WASF1 Antibody Target Details WASF1 Handling Images back to top
ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-WASF1 Antibody Target Details WASF1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-WASF1 Antibody Target Details WASF1 Application Details Handling back to top