anti-Human CARD11 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CARD11 Antibody (supplied by: Log in to see )

Antigen
Caspase Recruitment Domain Family, Member 11 (CARD11) Antibodies
  • CARD11
  • LOC100232755
  • BENTA
  • BIMP3
  • CARMA1
  • PPBL
  • 0610008L17Rik
  • 2410011D02Rik
  • caspase recruitment domain family, member 11
  • CARD11
  • LOC100232755
  • LOC100354145
  • LOC100363332
  • card11
  • Card11
Epitope
C-Term
26
9
6
4
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
70
37
8
5
5
1
1
1
1
1
Host
Goat
51
19
1
1
Clonality
Polyclonal
Conjugate
This CARD11 antibody is un-conjugated
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
66
38
34
17
5
5
4
3
2
2
1
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Catalog No. ABIN249816
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.079243 ABIN614918 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal
14.079243 ABIN364421 IHC (p) ELISA WB Goat AA 1160-1171 Log in to see Polyclonal
14.079243 ABIN214429 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
14.079243 ABIN4287879 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
14.079243 ABIN615803 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5554649 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN303197 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1732635 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal
1 ABIN604879 IHC (p) WB Rabbit AA 18-238 Log in to see Polyclonal
1 ABIN1732634 IHC (p) WB Rabbit IgG AA 18-238 Log in to see Polyclonal
1 ABIN1732636 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN715691 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN5559698 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN2768504 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2767986 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2771133 IHC (p) IHC ELISA WB Goat C-Term Log in to see Polyclonal
1 ABIN1882817 IHC (p) WB Rabbit Internal Region Log in to see Polyclonal

General

Antigen Caspase Recruitment Domain Family, Member 11 (CARD11) Antibodies
Epitope C-Term
(26), (9), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(70), (37), (8), (5), (5), (1), (1), (1), (1), (1)
Host Goat
(51), (19), (1), (1)
Clonality
Polyclonal
Conjugate This CARD11 antibody is un-conjugated
(1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(66), (38), (34), (17), (5), (5), (4), (3), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-CARD11 Antibody

Target Details CARD11 Application Details Handling References for anti-CARD11 antibody (ABIN249816) Images
Purification Immunogen affinity purified
Immunogen Peptide with sequence C-QRKTIWVDEDQL corresponding to C-Terminus according to NP_115791.3.

Target Details CARD11

Product Details anti-CARD11 Antibody Application Details Handling References for anti-CARD11 antibody (ABIN249816) Images back to top
Antigen
Alternative Name CARD11/CARMA1 (CARD11 Antibody Abstract)
Background Gene Symbol: CARD11
Gene ID 84433
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-CARD11 Antibody Target Details CARD11 Handling References for anti-CARD11 antibody (ABIN249816) Images back to top
Application Notes Western Blot 1 - 3 μg/mL, Immunohistochemistry 2 - 5 μg/mL, Immunohistochemistry-Paraffin 1:10 - 1:500, Peptide ELISA Detection limit 1:32000WB: Approx. 150+65 kDa band bands observed in Jurkat lysates (calculated MW of 133 kDa band according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (No picture). These bands are not observed in the non-transfected HEK293. An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human spleen shows nuclear staining.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CARD11 Antibody Target Details CARD11 Application Details References for anti-CARD11 antibody (ABIN249816) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-CARD11 antibody (ABIN249816)

Product Details anti-CARD11 Antibody Target Details CARD11 Application Details Handling Images back to top
Product cited in:

Rajasekaran, Kumar, Schuldt, Peterson, Vanhaesebroeck, Dixit, Thakar, Malarkannan: "Signaling by Fyn-ADAP via the Carma1-Bcl-10-MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells." in: Nature immunology, Vol. 14, Issue 11, pp. 1127-36, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Bertin, Wang, Guo, Jacobson, Poyet, Srinivasula, Merriam, DiStefano, Alnemri: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001

Images

Product Details anti-CARD11 Antibody Target Details CARD11 Application Details Handling References for anti-CARD11 antibody (ABIN249816) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (ABIN249816) Western Blot: CARD 11 Antibody [ABIN249816] - ABIN249816 staining (1ug/ml) of Human J...
Western Blotting (WB) image for anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (ABIN249816) Western Blot: CARD11/CARMA1 Antibody [ABIN249816] - (1ug/ml) of human Jurkat cells (R...
Western Blotting (WB) image for anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (ABIN249816) Western Blot: CARD11/CARMA1 Antibody [ABIN249816] - HEK293 overexpressing CARD11 and ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (ABIN249816) Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody [ABIN249816] - (2ug/ml) stainin...