You are viewing an incomplete version of our website. Please click to reload the website as full version.

Rat (Rattus) CD137 ELISA Kit

Recommended CD137 Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA Kits
  • TNFRSF9
  • zgc:136557
  • 4-1BB
  • CD137
  • CDw137
  • ILA
  • A930040I11Rik
  • AA408498
  • AI325004
  • Cd137
  • Ly63
  • tumor necrosis factor receptor superfamily, member 9
  • tumor necrosis factor receptor superfamily, member 9a
  • TNFRSF9
  • tnfrsf9a
  • LOC100348914
  • Tnfrsf9
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
16
8
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN2747925
$ 728.20
Plus shipping costs $45.00

Similar CD137 ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 1 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(16), (8)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details CD137 ELISA Kit

Target details Application Details Handling Images
Purpose Rat 4-1BB (TNFRSF9/CD137) ELISA Kit.
Sample Type Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate
Analytical Method Quantitative
Specificity The antibody pair provided in this kit recognizes Rat 4-1BB (TNFRSF9/CD137)
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details CD137 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name 4-1BB (TNFRSF9 ELISA Kit Abstract)
Background 4-1BB (TNFRSF9/CD137)
UniProt Q4W8J3
Pathways TCR Signaling

Application Details

Product Details CD137 ELISA Kit Target details Handling Images back to top
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details CD137 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months