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Rat (Rattus) CD137 ELISA Kit

Recommended CD137 Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA Kits
  • 4-1BB
  • A930040I11Rik
  • AA408498
  • AI325004
  • CD137
  • Cd137
  • CDw137
  • ILA
  • Ly63
  • TNFRSF9
  • zgc:136557
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
16
8
Methode Type
Sandwich ELISA
Application
ELISA
Supplier
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Catalog No. ABIN2747925
$ 715.00
Plus shipping costs $45.00

Similar CD137 ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 1 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(16), (8)
Methode Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details CD137 ELISA Kit

Target details Application Details Handling Images
Sample Type Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids
Detection Method Colorimetric
Specificity The antibody pair provided in this kit recognizes Rat 4-1BB (TNFRSF9/CD137)
Characteristics Quantitative
Components 96-well pre-coated strip plate (12x8)
Wash Buffer Concentrate (20x)
Standard
Sample Diluent Buffers
Biotin-labeled Detection Antibody
HRP-Streptavidin concentrate
TMB One-Step Substrate Reagent
Stop Solution
Cell Lysis Buffer (for cell/tissue lysate kits only)
Material not included Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details CD137 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name 4-1BB (TNFRSF9 ELISA Kit Abstract)
Background 4-1BB (TNFRSF9/CD137)
Pathways TCR Signaling

Application Details

Product Details CD137 ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Strip plates and additional reagents allow for use in multiple experiments.
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Plate 96 wells
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details CD137 ELISA Kit Target details Application Details Images back to top
Storage 4 °C,-20 °C,-80 °C
Storage Comment May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.