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anti-Dog (Canine) CD4 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Reactivity
Dog (Canine)
1198
691
240
66
51
45
25
23
22
21
19
15
14
9
9
8
7
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Mouse
1358
664
276
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
282
237
175
114
44
42
42
39
39
38
36
32
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Flow Cytometry (FACS), Immunoprecipitation (IP)
1802
436
395
352
309
296
179
136
100
70
33
28
27
27
22
18
14
11
10
9
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2166670
$ 721.43
Plus shipping costs $45.00

Similar anti-CD4 Antibodies

Application / Reactivity Dog (Canine)
ELISA 3 Antibodies
Flow Cytometry (FACS) 43 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 8 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Dog (Canine)
(1198), (691), (240), (66), (51), (45), (25), (23), (22), (21), (19), (15), (14), (9), (9), (8), (7), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1358), (664), (276), (4), (2), (1)
Clonality
Monoclonal
Conjugate This CD4 antibody is un-conjugated
(282), (237), (175), (114), (44), (42), (42), (39), (39), (38), (36), (32), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(1802), (436), (395), (352), (309), (296), (179), (136), (100), (70), (33), (28), (27), (27), (22), (18), (14), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling Images
Specificity Mouse anti Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) [Cobbold et al.1992 ] and identifies, by immunoprecipitation a 60 kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus (Moore et al. 1992).

CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarck et al. 2012 ). Similar reports have been made for cynomolgus monkey (Nam et al. 2000), pig (Saalmuller et al. 1987 , Pescowitz et al. 1990) as well as rat, chicken and human (reviewed Zuckermann 1999 ).

Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species (Moore et al. 1992).

Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages (Moore et al. 1992). Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4. Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by Rat anti Canine anti CD45RA clone CA4.1D3 in a manner analogous to that seen with human T cells (Moore et al. 1992).

Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoploriferative diseases. T lymphocytes in large granular lymophocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000 ). Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils (Tarrant et al. 2001). CD4 expression varies considerably between various canine leukemias (Villiers 2002 ).
Predicted Reactivity Dog
Characteristics Mouse Anti Dog Cd4
Protein Size: 463
Immunogen The immunogen for anti-CD4 antibody: canine Thymocytes
Isotype IgG1

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Mouse Anti Dog Cd4
Gene ID 403931
NCBI Accession NP_001003252
UniProt P33705
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
Format Liquid
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store at +4°C or at -20°C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.