|Application / Reactivity||Dog (Canine)|
|Flow Cytometry (FACS)||36 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunohistochemistry (IHC)||6 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||7 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Reactivity||Dog (Canine) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Immunohistochemistry (Acetone-fixed) (IHC (af)), Flow Cytometry (FACS), Immunoprecipitation (IP)
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling Images
Mouse anti Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) [Cobbold et al.1992 ] and identifies, by immunoprecipitation a 60 kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus (Moore et al. 1992).
CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarck et al. 2012 ). Similar reports have been made for cynomolgus monkey (Nam et al. 2000), pig (Saalmuller et al. 1987 , Pescowitz et al. 1990) as well as rat, chicken and human (reviewed Zuckermann 1999 ).
Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species (Moore et al. 1992).
Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages (Moore et al. 1992). Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4. Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by Rat anti Canine anti CD45RA clone CA4.1D3 in a manner analogous to that seen with human T cells (Moore et al. 1992).
Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoploriferative diseases. T lymphocytes in large granular lymophocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000 ). Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils (Tarrant et al. 2001). CD4 expression varies considerably between various canine leukemias (Villiers 2002 ).
Mouse Anti Dog Cd4
Protein Size: 463
|Immunogen||The immunogen for anti-CD4 antibody: canine Thymocytes|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||Mouse Anti Dog Cd4|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Store at +4°C or at -20°C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.