anti-Human CD4 antibody for Western Blotting

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Epitope
AA 420-457
93
65
23
21
19
19
15
15
14
13
10
9
8
8
8
6
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1250
655
234
78
68
41
36
28
27
26
22
21
19
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rabbit
1422
611
282
16
6
1
1
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
264
232
170
108
54
54
54
48
36
34
34
33
30
26
22
21
21
20
19
19
18
18
18
18
16
15
15
13
13
9
9
8
8
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1823
495
452
353
323
311
160
156
98
78
41
38
38
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN671376
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1469584 ABIN2787938 WB Rabbit C-Term Log in to see Polyclonal
3.1469584 ABIN1532559 IF IHC ELISA WB Rabbit IgG AA 401-450 Log in to see Polyclonal 1
3.1469584 ABIN439030 ICC IF IHC IHC (p) SimWes WB Rabbit C-Term, AA 400-458 Log in to see Polyclonal 12
3.1469584 ABIN1996695 ELISA WB Rabbit IgG AA 1-390 Log in to see 104 1
3.1469584 ABIN1996701 ELISA WB Rabbit IgG AA 1-390 Log in to see Polyclonal 1
3.1469584 ABIN4292753 IHC IHC (p) IHC (wm) WB Rabbit IgG Log in to see Polyclonal 2
3.1469584 ABIN2487040 WB Rabbit IgG C-Term Log in to see Polyclonal
3.1469584 ABIN1385275 IF (p) IHC (p) WB Rabbit IgG AA 320-370 Log in to see Polyclonal
3.1469584 ABIN2854871 WB Rabbit IgG Center Log in to see Polyclonal
3.1469584 ABIN3183771 ELISA IF IHC WB Rabbit IgG Ser213 Log in to see Polyclonal
3.1469584 ABIN1077935 ICC IHC IP WB Rabbit IgG AA 26-390 Log in to see Polyclonal
3.1469584 ABIN2452465 IHC WB Mouse IgG1 Log in to see 6E10
3.1469584 ABIN2452464 IHC WB Mouse IgG1 Log in to see 10B5
3.1469584 ABIN2452286 WB Mouse IgG1 Log in to see 9C1
3.1469584 ABIN2452331 IF WB Mouse IgG2a Log in to see 4A11
3.1469584 ABIN2452332 IF WB Mouse IgG1 Log in to see 9G7
3.1469584 ABIN2452287 WB Mouse IgG1 Log in to see 9B7
3.1469584 ABIN2452467 IHC WB Mouse IgG2b Log in to see 4F8
3.1469584 ABIN2452288 FACS WB Mouse IgG2b Log in to see 18E3
3.1469584 ABIN2452385 IF WB Mouse IgG2b Log in to see 3B4

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope AA 420-457
(93), (65), (23), (21), (19), (19), (15), (15), (14), (13), (10), (9), (8), (8), (8), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1250), (655), (234), (78), (68), (41), (36), (28), (27), (26), (22), (21), (19), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1422), (611), (282), (16), (6), (1), (1), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(264), (232), (170), (108), (54), (54), (54), (48), (36), (34), (34), (33), (30), (26), (22), (21), (21), (20), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (9), (9), (8), (8), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1823), (495), (452), (353), (323), (311), (160), (156), (98), (78), (41), (38), (38), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN671376) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from rat CD4
Isotype IgG

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN671376) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subcellular location: Cytoplasm

Synonyms: p55, W3/25, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen, CD4

Gene ID 24932
UniProt P05540
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN671376) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN671376) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CD4 antibody (ABIN671376)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Smit, Bester, van den Heever, Schlegel, Botes, Dohmen: "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?" in: Cell and tissue banking, 2015

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN671376) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclona...
Immunofluorescence (IF) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibo...
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgica...
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Mouse spleen cells probed with Rabbit Anti-CD4 Polyclonal Antibody, PE Conjugated fo...
Western Blotting (WB) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with at 1:5000 90min...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in...
'Independent Validation' Badge
Lot Number 130703
Method validated Immunohistochemistry
Positive Control Mouse spleen tissue
Negative Control Mouse muscle tissue
Notes Signal was detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antibody: CD4 Molecule (CD4)
  • Catalog number: ABIN671376
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 130703
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: Spleen tissue (specimen known to contain the target protein) from mouse.
  • Negative Control: Muslce tissue (specimen known to not contain the target protein) from mouse.
  • Primary antibody isotype control: Mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Mouse spleen treated with secondary antibody only (no primary antibody).
Protocol
  • Immunohistochemistry was performed manually.
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 10 min in 0.1M Borate buffer with 1 mM NaCl and 1 mM EDTA pH 8.0 for antigen retrieval.
  • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidases.
  • Sections were washed x 3 in Tris buffered saline (TBS).
  • Sections were blocked in 5 % normal donkey serum for 60 min at room temperature.
  • Sections were incubated with primary antibody diluted 1:200 in 5% normal donkey serum in TBS. Incubated at 4°C temperature overnight.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature..
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
  • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 sec at RT.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin for 20 sec.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
Experimental Notes - No experimental challenges noted.
Validation Images
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 1: Mouse spleen stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 2: Mouse muscle stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 3: Mouse spleen stained with isotype control antibody (brown) and counterstain...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 4: Mouse spleen stained with secondary antibody only (brown) and counterstaine...