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anti-Mouse (Murine) CD4 antibody for Biochemical Assay

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Reactivity
Mouse (Murine)
1198
690
240
66
52
45
25
23
22
21
19
15
14
9
9
8
7
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Rat
1359
663
276
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to Alexa Fluor 700
282
237
175
114
44
42
42
39
39
37
36
32
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Biochemical Assay (BCA)
1803
436
395
352
309
297
179
136
100
70
33
28
28
27
22
18
14
11
10
8
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2658056
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088740664 ABIN2658057 BCA Alexa Fluor 700 Rat IgG2b kappa Log in to see GK1-5 9
0.00088740664 ABIN2658058 BCA Alexa Fluor 700 Rat IgG2b kappa Log in to see GK1-5 11

Top referenced anti-CD4 antibody for Biochemical Assay

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 50 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 565 Antibodies
Functional Studies (Func) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 40 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 105 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 94 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 5 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1198), (690), (240), (66), (52), (45), (25), (23), (22), (21), (19), (15), (14), (9), (9), (8), (7), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Rat
(1359), (663), (276), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to Alexa Fluor 700
(282), (237), (175), (114), (44), (42), (42), (39), (39), (37), (36), (32), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Biochemical Assay (BCA)
(1803), (436), (395), (352), (309), (297), (179), (136), (100), (70), (33), (28), (28), (27), (22), (18), (14), (11), (10), (8), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN2658056) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions.
Clone RM4-5
Isotype IgG2a kappa

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN2658056) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN2658056) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN2658056) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD4 Antibody (ABIN2658056)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Zaborsky, Brunner, Wallner, Himly, Karl, Schwarzenbacher, Ferreira, Achatz: "Antigen aggregation decides the fate of the allergic immune response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 2, pp. 725-35, 2010

Shigeta, Matsumoto, Tedder, Lowe, Miyasaka, Hirata: "An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation." in: Blood, Vol. 112, Issue 13, pp. 4915-23, 2008

León-Ponte, Ahern, OConnell: "Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor." in: Blood, Vol. 109, Issue 8, pp. 3139-46, 2007

Matsumoto, Shigeta, Furukawa, Tanaka, Miyasaka, Hirata: "CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2499-506, 2007

Muraille, De Trez, Pajak, Torrentera, De Baetselier, Leo, Carlier: "Amastigote load and cell surface phenotype of infected cells from lesions and lymph nodes of susceptible and resistant mice infected with Leishmania major." in: Infection and immunity, Vol. 71, Issue 5, pp. 2704-15, 2003

Fan, Kouda, Nakamura, Takeuchi: "Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 226, Issue 11, pp. 1045-50, 2001

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule)  (Alexa Fluor 700) (ABIN2658056) anti-CD4 Molecule (CD4) antibody (Alexa Fluor 700)