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anti-Mouse (Murine) CD4 antibody for Immunohistochemistry (Zinc-fixed Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1189
666
252
72
61
43
34
24
22
22
19
17
16
15
11
11
10
10
9
7
6
6
5
5
4
2
2
2
1
1
1
1
Host
Rat
1359
626
272
9
4
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
281
239
171
106
47
45
43
36
36
35
34
32
25
25
21
21
20
19
18
18
18
18
16
15
13
13
12
10
8
8
7
6
6
6
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
1754
468
442
329
314
297
151
150
85
61
39
38
30
29
28
22
19
18
14
13
11
9
7
6
6
5
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN2689231
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689230 IHC (f) IHC (fro) FACS IHC (zinc) Rat IgG2a, kappa Log in to see RM4 6
1 ABIN1176846 BR CyTox IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 14
1 ABIN2689224 IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see RM4 13
1 ABIN2689225 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 15

Top referenced anti-CD4 antibody for Immunohistochemistry (Zinc-fixed Sections)

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 52 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 532 Antibodies
Functional Studies (Func) 22 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 59 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 125 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 67 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1189), (666), (252), (72), (61), (43), (34), (24), (22), (22), (19), (17), (16), (15), (11), (11), (10), (10), (9), (7), (6), (6), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1359), (626), (272), (9), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(281), (239), (171), (106), (47), (45), (43), (36), (36), (35), (34), (32), (25), (25), (21), (21), (20), (19), (18), (18), (18), (18), (16), (15), (13), (13), (12), (10), (8), (8), (7), (6), (6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
(1754), (468), (442), (329), (314), (297), (151), (150), (85), (61), (39), (38), (30), (29), (28), (22), (19), (18), (14), (13), (11), (9), (7), (6), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN2689231) Images
Brand BD Pharmingen™
Characteristics The H129.19 antibody reacts with the CD4 (L3T4) differentiation antigen expressed on thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. CD4 has also been detected on pluirpotent hematopoietic stem cells, bone marrow myeloid precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 is expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine lck. H129.19 mAb blocks binding of the anti-mouse CD4 mAbs Gk1.5 (Cat. No. 557307/553729) and RM4-5 (Cat. No. 553046/553047), but not RM4-4 (Cat. No. 553055) antibody. mAb H129.19 inhibits various responses of T helper cells to antigenic or mitogenic stimuli. Immunohistochemical staining of CD4 + T lymphocytes. Frozen sections of normal mouse spleen were reacted with H129.19 antibody. CD4+ T lymphocytes can be identified by the intense brown labeling of their cell surface membranes. Amplification 20X.

BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone H129.19 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen A.TH mouse CTL clone A15.1.17
Clone H129-19
Isotype IgG2a, kappa

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN2689231) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Synonyms: L3T4
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN2689231) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN2689231) Images back to top
Format Liquid
Concentration 62.5 μg/mL
Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD4 Antibody (ABIN2689231)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992

Wu, Scollay, Egerton, Pearse, Spangrude, Shortman: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, 1991

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, 1989

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Ghobrial, Boublik, Winn, Auchincloss: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, 1989

Pont, Régnier-Vigouroux, Marchetto, Pierres: "Accessory molecules and T cell activation. II. Antibody binding to L3T4a inhibits Ia-independent mouse T cell proliferation." in: European journal of immunology, Vol. 17, Issue 3, pp. 429-32, 1987

Naquet, Malissen, Bekkhoucha, Pont, Pierres, Hood, Pierres: "L3T4 but not LFA-1 participates in antigen presentation by Ak-positive L-cell transformants." in: Immunogenetics, Vol. 22, Issue 3, pp. 247-56, 1985

Pierres, Naquet, Van Agthoven, Bekkhoucha, Denizot, Mishal, Schmitt-Verhulst, Pierres et al.: "A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 6, pp. 2775-82, 1984