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anti-Mouse (Murine) CD4 antibody for Immunoprecipitation

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Reactivity
Mouse (Murine)
1164
654
241
72
56
41
26
23
22
21
19
15
15
15
11
10
9
8
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Rat
1330
623
273
8
4
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
274
237
170
106
45
44
42
35
35
35
33
33
24
22
21
20
20
18
17
17
17
17
16
15
15
13
13
8
8
7
7
7
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
1742
424
400
333
304
298
180
139
115
67
37
28
28
27
22
18
14
14
11
9
7
6
6
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN614792
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008947584 ABIN2660685 ELISA (Capture) IF IP WB APC-Cy7 Rat IgG2a kappa Log in to see RM4-5 8
0.0008947584 ABIN2661074 BR Dep FACS IP IHC Biotin Rat IgG2b kappa Log in to see GK1-5 9
0.0008947584 ABIN2661703 BR FACS IF ICFC IP IHC FITC Rat IgG2a kappa Log in to see RM4-5 8
0.0008947584 ABIN2661704 FACS IF IP IHC FITC Rat IgG2a kappa Log in to see H129-19 6
0.0008947584 ABIN2661709 BR FACS IF ICFC IP IHC FITC Rat IgG2b kappa Log in to see RM4-4 4
0.0008947584 ABIN2658972 FACS IP IHC WB PE-Cy5 Rat IgG2a kappa Log in to see RM4-5 8
0.0008947584 ABIN343704 FACS Func ICC IHC (f) IP Rat IgG2b Extracellular Log in to see GK1-5 10
0.0008947584 ABIN234619 FACS IFA IP Biotin Rat IgG2b Log in to see GK1-5 4
0.0008947584 ABIN1176974 BR IHC (fro) FACS IP Rat IgG2b, kappa Log in to see GK1-5 9
0.0008947584 ABIN135102 FACS IP IHC Biotin Rat IgG2b Log in to see GK1-5 2
0.0008947584 ABIN1176846 BR CyTox IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 14
0.0008947584 ABIN2689231 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 9
0.0008947584 ABIN2747750 IP ELISA WB Rabbit IgG AA 270-320, N-Term Log in to see Polyclonal
0.0008947584 ABIN509566 FACS Func ICC IHC (f) IP FITC Rat IgG2b Extracellular Log in to see GK1-5 9
0.0008947584 ABIN457367 FACS Func ICC IHC (f) IP PE Rat IgG2b Extracellular Log in to see GK1-5 9
0.0008947584 ABIN457307 FACS Func ICC IHC (f) IP APC Rat IgG2b Extracellular Log in to see GK1-5 9
0.0008947584 ABIN263597 FACS IF IP Biotin Rat IgG2b Log in to see GK1-5
0.0008947584 ABIN135099 FACS IP IHC Rat IgG2b Log in to see GK1-5 2
0.0008947584 ABIN135105 FACS IP IHC Rat IgG2b Log in to see GK1-5 2
0.0008947584 ABIN2480261 IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4

Top referenced anti-CD4 antibody for Immunoprecipitation

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 49 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 528 Antibodies
Functional Studies (Func) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 44 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 25 Antibodies
Immunohistochemistry (IHC) 108 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 69 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 5 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1164), (654), (241), (72), (56), (41), (26), (23), (22), (21), (19), (15), (15), (15), (11), (10), (9), (8), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Rat
(1330), (623), (273), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(274), (237), (170), (106), (45), (44), (42), (35), (35), (35), (33), (33), (24), (22), (21), (20), (20), (18), (17), (17), (17), (17), (16), (15), (15), (13), (13), (8), (8), (7), (7), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
(1742), (424), (400), (333), (304), (298), (180), (139), (115), (67), (37), (28), (28), (27), (22), (18), (14), (14), (11), (9), (7), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN614792) Images
Specificity This antibody reacts with an extracellular epitope of Mouse CD4 transmembrane glycoprotein (55 kDa).
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Protein A Chromatography
Immunogen Mouse CTL clone V4 cells
Clone GK1-5
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN614792) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 12504, 10090
UniProt P06332
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN614792) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN614792) Images back to top
Concentration 1.0 mg/mL
Buffer Azide Free PBS, pH ~7.4, 0.2 μm filter sterilized
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 Antibody (ABIN614792)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Felix, Donermeyer, Horvath, Walters, Gross, Suri, Allen: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, 2007

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, 1997

Gavett, Chen, Finkelman, Wills-Karp: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, 1994

Godfrey, Kennedy, Gately, Hakimi, Hubbard, Zlotnik: "IL-12 influences intrathymic T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2729-35, 1994

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992

Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983

Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, 1983