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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||2 Antibodies|
|Cytotoxicity Test (CyTox)||4 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||146 Antibodies|
|Functional Studies (Func)||6 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies|
|Immunofluorescence (IF)||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||9 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||31 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies|
|Immunoprecipitation (IP)||18 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS)
|8 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN1302491) Images
|Specificity||The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).|
|Purification||Purified from cell culture supernatant by protein-A affinity chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Immunogen||MLR generated rat Th cells|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN1302491) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell differenciation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN1302491) Images back to top
Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.
Working concentrations should be determined by the investigator.
CD and Related Antigens & MHC (Rodent)
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN1302491) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD4 Antibody (ABIN1302491)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
|Product cited in:||
Viel, Lemarié, Benkirane et al.: "Immune regulation and vascular inflammation in genetic hypertension." in: American journal of physiology. Heart and circulatory physiology, Vol. 298, Issue 3, pp. H938-44, 2010 (PubMed).
Baba, Iwasaki, Maruoka et al.: "Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2999-3006, 2008 (PubMed).
Ramiro-Puig, Pérez-Cano, Ramos-Romero et al.: "Intestinal immune system of young rats influenced by cocoa-enriched diet." in: The Journal of nutritional biochemistry, Vol. 19, Issue 8, pp. 555-65, 2008 (PubMed).
Gelderman, Hultqvist, Holmberg et al.: "T cell surface redox levels determine T cell reactivity and arthritis susceptibility." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 34, pp. 12831-6, 2006 (PubMed).
Baba, Ishizu, Iwasaki et al.: "CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype." in: Blood, Vol. 107, Issue 5, pp. 2004-12, 2006 (PubMed).
Yan, Devos, Yu et al.: "Pathogenesis of autoimmunity after xenogeneic thymus transplantation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5936-46, 2003 (PubMed).
Salomon, Netzer, Wildbaum et al.: "Targeting the function of IFN-gamma-inducible protein 10 suppresses ongoing adjuvant arthritis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 5, pp. 2685-93, 2002 (PubMed).
Dragun, Lukitsch, Tullius et al.: "Inhibition of intercellular adhesion molecule-1 with antisense deoxynucleotides prolongs renal isograft survival in the rat." in: Kidney international, Vol. 54, Issue 6, pp. 2113-22, 1999 (PubMed).