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anti-Rat (Rattus) CD4 antibody for Immunocytochemistry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Epitope
Extracellular
77
40
22
22
13
13
13
10
8
8
7
7
7
7
6
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1198
694
239
69
52
45
25
23
22
21
19
15
14
12
9
8
7
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Mouse
1361
667
276
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
284
239
176
114
44
42
42
39
39
38
36
32
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS)
1808
436
399
356
309
300
179
136
99
70
33
28
28
27
22
18
14
11
10
9
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1302491
$ 196.35
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN2661707 ELISA (Capture) ELISPOT (Capture) ICC Neut IHC FITC Mouse IgG2a kappa Log in to see OX-35 2
0.0009962841 ABIN2506709 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN2749065 ICC FACS IHC PE Mouse IgG2a Log in to see OX-35 10
0.0009962841 ABIN2749064 ICC FACS IHC FITC Mouse IgG2a Log in to see OX-35 9
0.0009962841 ABIN2749063 ICC FACS IHC APC Mouse IgG2a Log in to see OX-35 9
0.0009962841 ABIN3199810 FACS ICC IHC APC Mouse IgG2a Log in to see OX-35
0.0009962841 ABIN3199812 FACS ICC IHC PE Mouse IgG2a Log in to see OX-35
0.0009962841 ABIN3199811 FACS ICC IHC FITC Mouse IgG2a Log in to see OX-35
0.0009962841 ABIN2506618 ICC IF FITC Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN3199809 FACS ICC IHC Mouse IgG2a Log in to see OX-35
0.0009962841 ABIN1112855 ICC FACS WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN4292746 ICC IF IHC IHC (p) SimWes WB Rabbit AA 400-458, C-Term Log in to see Polyclonal
0.0009962841 ABIN3037243 ICC FACS IF IHC Mouse IgG2a Log in to see
0.0009962841 ABIN3037244 ICC FACS IF IHC FITC Mouse IgG2a Log in to see
0.0009962841 ABIN3037245 ICC FACS IF IHC PE Mouse IgG2a Log in to see
0.0009962841 ABIN3037246 ICC FACS IF IHC APC Mouse IgG2a Log in to see
0.0009962841 ABIN1854860 ICC FACS IF WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN1854063 ICC FACS IF IP FITC Mouse IgG2a Log in to see
0.0009962841 ABIN1854064 ICC FACS IF IP Biotin Mouse IgG2a Log in to see
0.0009962841 ABIN1854065 ICC FACS IF IP PE Mouse IgG2a Log in to see

Top referenced anti-CD4 antibody for Immunocytochemistry

Similar anti-CD4 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
Cytotoxicity Test (CyTox) 4 Antibodies
ELISA 15 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 174 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 9 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Neutralization (Neut) 1 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Extracellular
(77), (40), (22), (22), (13), (13), (13), (10), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1198), (694), (239), (69), (52), (45), (25), (23), (22), (21), (19), (15), (14), (12), (9), (8), (7), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1361), (667), (276), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(284), (239), (176), (114), (44), (42), (42), (39), (39), (38), (36), (32), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS)
(1808), (436), (399), (356), (309), (300), (179), (136), (99), (70), (33), (28), (28), (27), (22), (18), (14), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN1302491) Images
Specificity The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen MLR generated rat Th cells
Clone OX-35
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN1302491) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell differenciation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN1302491) Images back to top
Application Notes Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens & MHC (Rodent)

Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN1302491) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD4 Antibody (ABIN1302491)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Viel, Lemarié, Benkirane, Paradis, Schiffrin: "Immune regulation and vascular inflammation in genetic hypertension." in: American journal of physiology. Heart and circulatory physiology, Vol. 298, Issue 3, pp. H938-44, 2010

Ramiro-Puig, Pérez-Cano, Ramos-Romero, Pérez-Berezo, Castellote, Permanyer, Franch, Izquierdo-Pulido, Castell: "Intestinal immune system of young rats influenced by cocoa-enriched diet." in: The Journal of nutritional biochemistry, Vol. 19, Issue 8, pp. 555-65, 2008

Baba, Iwasaki, Maruoka, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara, Ishizu: "Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2999-3006, 2008

Baba, Ishizu, Iwasaki, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara: "CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype." in: Blood, Vol. 107, Issue 5, pp. 2004-12, 2006

Gelderman, Hultqvist, Holmberg, Olofsson, Holmdahl: "T cell surface redox levels determine T cell reactivity and arthritis susceptibility." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 34, pp. 12831-6, 2006

Yan, Devos, Yu, Xia, Rutgeerts, Goebels, Segers, Lin, Vandeputte, Waer: "Pathogenesis of autoimmunity after xenogeneic thymus transplantation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5936-46, 2003

Salomon, Netzer, Wildbaum, Schif-Zuck, Maor, Karin: "Targeting the function of IFN-gamma-inducible protein 10 suppresses ongoing adjuvant arthritis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 5, pp. 2685-93, 2002

Dragun, Lukitsch, Tullius, Qun, Park, Schneider, Luft, Haller: "Inhibition of intercellular adhesion molecule-1 with antisense deoxynucleotides prolongs renal isograft survival in the rat." in: Kidney international, Vol. 54, Issue 6, pp. 2113-22, 1999