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anti-Rat (Rattus) CD4 antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Rat (Rattus)
1189
667
251
72
61
43
34
24
22
22
19
17
16
15
11
11
10
10
9
7
6
6
5
5
4
2
2
2
1
1
1
1
Host
Mouse
1358
627
272
9
4
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
281
239
171
106
47
45
43
36
36
35
34
32
25
25
21
21
20
19
18
18
18
18
16
15
13
13
12
10
8
8
7
6
6
6
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Flow Cytometry (FACS)
1754
468
442
330
314
298
151
150
85
61
39
38
30
29
28
22
19
18
14
14
11
9
7
6
6
5
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN2165077
$ 168.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2165002 IHC (af) IHC (fp) FACS Mouse IgG1 Log in to see
1 ABIN2166662 IHC (af) IHC (fp) FACS Mouse IgG1 Log in to see
1 ABIN2165076 IHC (af) IHC (fp) FACS Mouse IgG1 Log in to see

Similar anti-CD4 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
Cytotoxicity Test (CyTox) 4 Antibodies
ELISA 16 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 174 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 9 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 21 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Rat (Rattus)
(1189), (667), (251), (72), (61), (43), (34), (24), (22), (22), (19), (17), (16), (15), (11), (11), (10), (10), (9), (7), (6), (6), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1358), (627), (272), (9), (4), (1)
Clonality
Monoclonal
Conjugate This CD4 antibody is un-conjugated
(281), (239), (171), (106), (47), (45), (43), (36), (36), (35), (34), (32), (25), (25), (21), (21), (20), (19), (18), (18), (18), (18), (16), (15), (13), (13), (12), (10), (8), (8), (7), (6), (6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Flow Cytometry (FACS)
(1754), (468), (442), (330), (314), (298), (151), (150), (85), (61), (39), (38), (30), (29), (28), (22), (19), (18), (14), (14), (11), (9), (7), (6), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling Images
Specificity Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction.
Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whiteland et al. 1995).
Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes
Predicted Reactivity Rat
Characteristics Mouse Anti Rat Cd4 (DOMAIN 1)
Protein Size: 457
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen The immunogen for anti-Cd4 antibody: rat Thymocyte Membrane Glycoproteins
Isotype IgG1

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cd4 (CD4 Antibody Abstract)
Background Mouse Anti Rat Cd4 (DOMAIN 1)
Gene ID 24932
NCBI Accession NP_036837
UniProt P05540
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
Application Notes Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 106 cells in 100 μL.. Immunohistology - Paraffin:PLP fixation is recommended for optimal results, see Whiteland et al. for details
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
Format Liquid
Handling Advice Avoid repeat freeze-thaw cycles.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C,-20 °C
Storage Comment Store at +4 °C for one month or at -20 °C for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (ABIN2165077) anti-CD4 Molecule (CD4) antibody
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (ABIN2165077) anti-CD4 Molecule (CD4) antibody (Image 2)
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (ABIN2165077) anti-CD4 Molecule (CD4) antibody (Image 3)
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (ABIN2165077) anti-CD4 Molecule (CD4) antibody (Image 4)