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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||2 Antibodies|
|Cytotoxicity Test (CyTox)||4 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||174 Antibodies|
|Functional Studies (Func)||6 Antibodies|
|Immunocytochemistry (ICC)||15 Antibodies|
|Immunofluorescence (IF)||8 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||9 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||49 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||35 Antibodies|
|Immunoprecipitation (IP)||21 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Flow Cytometry (FACS)
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling Images
Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction.
Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whiteland et al. 1995).
Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes
Mouse Anti Rat Cd4 (DOMAIN 1)
Protein Size: 457
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||The immunogen for anti-Cd4 antibody: rat Thymocyte Membrane Glycoproteins|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling Images back to top
|Alternative Name||Cd4 (CD4 Antibody Abstract)|
|Background||Mouse Anti Rat Cd4 (DOMAIN 1)|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
|Application Notes||Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 106 cells in 100 μL.. Immunohistology - Paraffin:PLP fixation is recommended for optimal results, see Whiteland et al. for details|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
Avoid repeat freeze-thaw cycles.
Should this product contain a precipitate we recommend microcentrifugation before use.
|Storage||4 °C,-20 °C|
Store at +4 °C for one month or at -20 °C for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
ImagesProduct Details anti-CD4 Antibody Target Details CD4 Application Details Handling back to top