anti-Rat (Rattus) CD4 antibody for Immunoprecipitation

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Epitope
Domain 2
66
64
21
18
15
15
15
13
12
10
9
8
8
8
8
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1172
619
233
68
67
40
36
22
22
19
18
18
16
15
15
12
12
10
10
9
6
5
5
5
4
3
2
2
1
1
1
1
Host
Mouse
1323
573
273
10
4
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
258
222
166
100
48
48
46
40
36
34
33
30
27
23
21
21
19
19
18
18
18
18
15
15
14
13
13
13
11
8
7
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
1675
468
431
332
309
288
152
142
87
61
39
38
30
30
23
19
18
14
14
11
9
7
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN120380
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120383 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 8
1 ABIN2661701 FACS IP IHC WB FITC Mouse IgG1 kappa Log in to see W3-25 6
1 ABIN2480228 IHC (fro) FACS IP Mouse IgG2a Domain 1 Log in to see OX-38 4
1 ABIN120381 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 1
1 ABIN2664660 BR IP IHC ELISA WB Mouse IgG1 kappa Log in to see W3-25 6
1 ABIN2480233 FACS IF IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 4
1 ABIN206563 FACS IHC (fro) IF IP ELISA Mouse IgG2a Log in to see RIV6
1 ABIN2689228 BR IHC (fro) FACS IP Mouse IgG2a, kappa Log in to see OX 9
1 ABIN2689227 IHC (fro) FACS IP Mouse IgG2a, kappa Log in to see OX 8
1 ABIN206159 FACS IHC (fro) IP Mouse IgG2a Log in to see
1 ABIN213133 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-38
1 ABIN2225958 FACS IP IHC Mouse IgG2a Log in to see 3H733
1 ABIN210173 FACS IHC (fro) IP Mouse IgG2a Log in to see
1 ABIN1854064 FACS IF/ICC IP Biotin Mouse IgG2a Log in to see
1 ABIN1854063 FACS IF/ICC IP FITC Mouse IgG2a Log in to see
1 ABIN1854065 FACS IF/ICC IP PE Mouse IgG2a Log in to see
1 ABIN5558707 ELISA IHC IP WB Biotin Rabbit C-Term Log in to see Polyclonal
1 ABIN2900378 FACS IF/ICC IP APC Mouse IgG2a Log in to see
1 ABIN2900379 FACS IF/ICC IP PerCP Mouse IgG2a Log in to see

Top referenced anti-CD4 antibody for Immunoprecipitation

Similar anti-CD4 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
Cytotoxicity Test (CyTox) 4 Antibodies
ELISA 15 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 156 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 20 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain 2
(66), (64), (21), (18), (15), (15), (15), (13), (12), (10), (9), (8), (8), (8), (8), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1172), (619), (233), (68), (67), (40), (36), (22), (22), (19), (18), (18), (16), (15), (15), (12), (12), (10), (10), (9), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(1323), (573), (273), (10), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(258), (222), (166), (100), (48), (48), (46), (40), (36), (34), (33), (30), (27), (23), (21), (21), (19), (19), (18), (18), (18), (18), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(1675), (468), (431), (332), (309), (288), (152), (142), (87), (61), (39), (38), (30), (30), (23), (19), (18), (14), (14), (11), (9), (7), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120380) Images
Purification Affinity chromatography on Protein G
Immunogen MLR generated rat T helper lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Clone OX-35
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120380) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 10116
UniProt P05540
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN120380) Images back to top
Application Notes Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN120380) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD4 antibody (ABIN120380)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Wang, Wu, Shieh, Wen, Ling: "Immunohistochemical study of amoeboid microglial cells in fetal rat brain." in: Journal of anatomy, Vol. 189 ( Pt 3), pp. 567-74, 1997

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, 1985