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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||2 Antibodies|
|Cytotoxicity Test (CyTox)||4 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||146 Antibodies|
|Functional Studies (Func)||6 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies|
|Immunofluorescence (IF)||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||9 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||31 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies|
|Immunoprecipitation (IP)||18 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Epitope||Domain 2 Alternatives|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
|8 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN120383) Images
|Purification||Affinity chromatography on Protein G|
|Immunogen||MLR generated rat T helper lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN120383) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN120383) Images back to top
Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN120383) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD4 Antibody (ABIN120383)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Wang, Wu, Shieh et al.: "Immunohistochemical study of amoeboid microglial cells in fetal rat brain." in: Journal of anatomy, Vol. 189 ( Pt 3), pp. 567-74, 1997 (PubMed).
Charteris, Lightman: "In vivo lymphokine production in experimental autoimmune uveoretinitis." in: Immunology, Vol. 78, Issue 3, pp. 387-92, 1993 (PubMed).
Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).
Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, 1985 (PubMed).
Tellides, Dallman, Kupiec-Weglinski et al.: "Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy." in: Transplantation proceedings, Vol. 19, Issue 5, pp. 4231-3, 1987 (PubMed).
Hayosh, Swanborg: "Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 11, pp. 3771-5, 1987 (PubMed).
Signore, Parman, Pozzilli et al.: "Detection of activated lymphocytes in endocrine pancreas of BB/W rats by injection of 123I-interleukin-2: an early sign of type 1 diabetes." in: Lancet, Vol. 2, Issue 8558, pp. 537-40, 1987 (PubMed).
Paterson, Jefferies, Green et al.: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988 (PubMed).