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|Antigen||CD4 Molecule (CD4) Antibodies|
|Epitope||Domain 2 Alternatives|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
|2 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN120380) Images
|Purification||Affinity chromatography on Protein G|
|Immunogen||MLR generated rat T helper lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN120380) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN120380) Images back to top
Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN120380) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD4 antibody (ABIN120380)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Wang, Wu, Shieh, Wen, Ling: "Immunohistochemical study of amoeboid microglial cells in fetal rat brain." in: Journal of anatomy, Vol. 189 ( Pt 3), pp. 567-74, 1997
Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, 1985