anti-Human CD80 antibody for Flow Cytometry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • CD8
  • Leu2
  • MAL
  • p32
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • CD80 molecule
  • CD8a molecule
  • CD80 antigen
  • Cd80 molecule
  • CD80
  • CD8A
  • Cd80
Epitope
AA 35-242
24
21
17
15
15
15
13
11
10
10
8
8
6
5
5
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
437
272
48
10
10
7
6
3
2
2
1
Host
Mouse
393
115
97
85
41
18
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
75
71
50
25
20
18
15
14
12
11
10
10
8
8
8
8
7
5
5
5
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut)
509
204
134
127
116
70
62
44
31
28
26
8
8
7
7
7
6
6
5
5
4
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN4900274
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.326658 ABIN4900275 ELISA (Capture) FACS IHC Neut Mouse IgG1 AA 35-242 Log in to see 37711 3
14.326658 ABIN4897474 FACS Fluorescein Mouse IgG1 AA 35-242 Log in to see 37711 3
14.326658 ABIN4897476 FACS PE Mouse IgG1 AA 35-242 Log in to see 37711 3
14.326658 ABIN4897477 FACS PE Mouse IgG1 AA 35-242 Log in to see 37711 3
14.326658 ABIN4897475 FACS Fluorescein Mouse IgG1 AA 35-242 Log in to see 37711 3
14.326658 ABIN1997277 FACS ELISA Mouse IgG1 AA 1-242 Log in to see 01 1
14.326658 ABIN269072 CyTOF FACS IP Mouse IgG1 Log in to see MEM-233 1
14.326658 ABIN4282682 FACS IHC IHC (p) WB Mouse IgG2b kappa Log in to see 62N3G8 1
14.326658 ABIN259746 FACS Allophycocyanin Mouse IgG1 Log in to see MEM-233 1
14.326658 ABIN457391 FACS IP PerCP Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
14.326658 ABIN94239 FACS IP Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
14.326658 ABIN94240 FACS IP FITC Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
14.326658 ABIN94241 FACS IP APC Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
14.326658 ABIN192195 FACS IP PE Mouse IgG1 Extracellular Domain Log in to see MEM-233 9
14.326658 ABIN2144693 FACS FITC Mouse IgG1 Log in to see 4A2D10-4 1
14.326658 ABIN2144969 FACS PE Mouse IgG1 Log in to see 4A2D10-4 1
14.326658 ABIN2144971 FACS PE Mouse IgG1 Log in to see 4A2D10-4 1
14.326658 ABIN2144691 FACS FITC Mouse IgG1 Log in to see 4A2D10-4 1
14.326658 ABIN2663591 FACS PE Mouse IgG1 kappa Log in to see 2D10 10
14.326658 ABIN2657477 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see 2D10 7

Top referenced anti-CD80 antibody for Flow Cytometry

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General

Antigen CD80 Antibodies
Epitope AA 35-242
(24), (21), (17), (15), (15), (15), (13), (11), (10), (10), (8), (8), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(437), (272), (48), (10), (10), (7), (6), (3), (2), (2), (1)
Host Mouse
(393), (115), (97), (85), (41), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(75), (71), (50), (25), (20), (18), (15), (14), (12), (11), (10), (10), (8), (8), (8), (8), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut)
(509), (204), (134), (127), (116), (70), (62), (44), (31), (28), (26), (8), (8), (7), (7), (7), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN4900274) Images
Specificity Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80, Val35-Asn242 (predicted), Accession # P33681
Clone 37711
Isotype IgG1

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN4900274) Images back to top
Antigen
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Background Alternative Names: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 Molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt P33681
Research Area Stem Cells, Adaptive Immunity, Hematopoietic Progenitors, Surface Receptors of Immune Cells, CD Antigens
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling ProductDetails: References for anti-CD80 antibody (ABIN4900274) Images back to top
Application Notes Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
Neutralization 0.2-1 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 2-8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details ProductDetails: References for anti-CD80 antibody (ABIN4900274) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-CD80 antibody (ABIN4900274)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Ribot, Debarros, Mancio-Silva, Pamplona, Silva-Santos: "B7-CD28 costimulatory signals control the survival and proliferation of murine and human ?? T cells via IL-2 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 3, pp. 1202-8, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Weissmüller, Semmler, Kalinke, Christians, Müller-Berghaus, Waibler: "ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation." in: Blood, Vol. 119, Issue 26, pp. 6268-77, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Kosaka, Yashiro, Sakate, Hirakawa: "A synergistic antitumor effect of interleukin-2 addition with CD80 immunogene therapy for peritoneal metastasis of gastric carcinoma." in: Digestive diseases and sciences, Vol. 52, Issue 8, pp. 1946-53, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN4900274) back to top
Supplier Images
Neutralization (Neut) image for anti-CD80 antibody (CD80) (AA 35-242) (ABIN4900274) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Human B7-1/CD80 Antibody.
Flow Cytometry (FACS) image for anti-CD80 antibody (CD80) (AA 35-242) (ABIN4900274) Detection of B7-1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt'...
Immunohistochemistry (IHC) image for anti-CD80 antibody (CD80) (AA 35-242) (ABIN4900274) B7-1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedde...