anti-Human CD80 antibody for Western Blotting

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • CD8
  • Leu2
  • MAL
  • p32
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • CD80 molecule
  • CD8a molecule
  • CD80 antigen
  • Cd80 molecule
  • CD80
  • CD8A
  • Cd80
Epitope
Internal Region
24
22
17
15
15
15
13
11
10
9
8
8
6
5
5
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
446
282
48
11
10
7
6
3
2
2
1
1
Host
Goat
404
115
102
91
41
18
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
76
74
53
26
20
18
15
14
13
12
10
10
8
8
8
8
7
7
7
5
5
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
526
203
139
137
122
81
64
45
31
31
26
9
8
7
7
7
6
6
5
5
4
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN615921
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN4282682 FACS IHC IHC (p) WB Mouse IgG2b kappa Log in to see 62N3G8 1
3.3487515 ABIN3042612 WB Rabbit IgG AA 57-71, N-Term Log in to see Polyclonal
3.3487515 ABIN2789329 WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN687142 IF (p) IHC (p) WB Rabbit IgG AA 90-140 Log in to see Polyclonal
3.3487515 ABIN2453864 IHC WB Mouse IgG2a Log in to see 3F1
3.3487515 ABIN2464853 ELISA WB Goat Internal Region Log in to see Polyclonal
3.3487515 ABIN4255211 ELISA WB Goat Log in to see Polyclonal 1
3.3487515 ABIN651210 FACS WB Rabbit Ig AA 260-288, C-Term Log in to see Polyclonal
3.3487515 ABIN2452895 IF WB Mouse IgG1 Log in to see 10D8
3.3487515 ABIN2452896 IF WB Mouse IgG1 Log in to see 7E4
3.3487515 ABIN2452898 WB Mouse IgG1 Log in to see 7H3
3.3487515 ABIN2452901 IHC WB Mouse IgG2b Log in to see 2H1
3.3487515 ABIN2452897 IF WB Mouse IgG1 Log in to see 6H7
3.3487515 ABIN2452902 FACS IF WB Mouse IgG1 Log in to see 1E9
3.3487515 ABIN2452893 IF WB Mouse IgG1 Log in to see 9H1
3.3487515 ABIN2452894 IF WB Mouse IgG1 Log in to see 7H7
3.3487515 ABIN4282673 ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 2E5
3.3487515 ABIN2681733 IHC (p) ELISA WB Rabbit IgG AA 1-242 Log in to see Polyclonal
3.3487515 ABIN2561749 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.3487515 ABIN3028553 FACS ELISA WB Rabbit Ig Fraction AA 260-288 Log in to see Polyclonal

Top referenced anti-CD80 antibody for Western Blotting

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Application / Reactivity Human
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ELISA 111 Antibodies
ELISA (Capture) 2 Antibodies
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Flow Cytometry (FACS) 271 Antibodies
Functional Studies (Func) 9 Antibodies
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Immunofluorescence (IF) 58 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
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General

Antigen CD80 Antibodies
Epitope Internal Region
(24), (22), (17), (15), (15), (15), (13), (11), (10), (9), (8), (8), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(446), (282), (48), (11), (10), (7), (6), (3), (2), (2), (1), (1)
Host Goat
(404), (115), (102), (91), (41), (18)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(76), (74), (53), (26), (20), (18), (15), (14), (13), (12), (10), (10), (8), (8), (8), (8), (7), (7), (7), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(526), (203), (139), (137), (122), (81), (64), (45), (31), (31), (26), (9), (8), (7), (7), (7), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN615921) Images
Sequence C-HLRVNQTFNW NTTK
Specificity Recognizes CD80.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_005182.1. Genename: CD80

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN615921) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. Cell Distribution: CD80 is rapidly induced on the surface of activated B cells, T cells and monocytes and is also expressed at high levels on dendritic cells.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene ID 941
NCBI Accession NP_005182
UniProt P33681
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling ProductDetails: References for anti-CD80 antibody (ABIN615921) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details ProductDetails: References for anti-CD80 antibody (ABIN615921) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD80 antibody (ABIN615921)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Background publications

Stephan, Ponomarev, Brentjens, Chang, Dobrenkov, Heller, Sadelain: "T cell-encoded CD80 and 4-1BBL induce auto- and transcostimulation, resulting in potent tumor rejection." in: Nature medicine, Vol. 13, Issue 12, pp. 1440-9, 2007

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN615921) back to top
Supplier Images
Western Blotting (WB) image for anti-CD80 antibody (CD80) (Internal Region) (ABIN615921) AP23702PU-N CD80 antibody staining of Human Node lysate at 1 µg/ml (35µg protein in R...