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anti-Mouse (Murine) CD80 antibody for Enzyme Immunoassay

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • B71
  • BB1
  • CD8
  • Cd28l
  • CD28LG
  • CD28LG1
  • LAB7
  • Leu2
  • Ly-53
  • Ly53
  • MAL
  • MIC17
  • p32
  • TSA1
Epitope
AA 45-63
24
16
16
15
15
14
13
13
11
6
5
5
5
5
3
2
2
2
2
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
408
271
49
17
10
9
8
3
2
2
2
Host
Rabbit
380
109
98
73
50
17
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
78
76
51
26
17
15
15
11
11
10
7
7
6
6
6
6
6
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Western Blotting (WB)
489
206
116
110
102
66
46
44
33
22
17
16
14
7
7
7
6
5
5
4
2
2
2
1
1
1
Supplier
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Catalog No. ABIN492862
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870063 ABIN116382 EIA Biotin Rabbit Log in to see Polyclonal
0.0008870063 ABIN116381 EIA Biotin Rabbit Log in to see Polyclonal
0.0008870063 ABIN116384 EIA WB Rabbit Log in to see Polyclonal
0.0008870063 ABIN116383 EIA WB Rabbit Log in to see Polyclonal

Top referenced anti-CD80 antibody for Enzyme Immunoassay

Similar anti-CD80 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies 6 Antibodies
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 5 Antibodies 21 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 10 Antibodies
Blocking Antibody (Inhibition) 2 Antibodies
Depletion (Dep) 2 Antibodies

General

Antigen CD80 Antibodies
Epitope AA 45-63
(24), (16), (16), (15), (15), (14), (13), (13), (11), (6), (5), (5), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(408), (271), (49), (17), (10), (9), (8), (3), (2), (2), (2)
Host Rabbit
(380), (109), (98), (73), (50), (17)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(78), (76), (51), (26), (17), (15), (15), (11), (11), (10), (7), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB), Enzyme Immunoassay (EIA), Western Blotting (WB)
(489), (206), (116), (110), (102), (66), (46), (44), (33), (22), (17), (16), (14), (7), (7), (7), (6), (5), (5), (4), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN492862) Images
Sequence YDH FID CFI EYG DAH VS
Specificity This antibody detects CD80 at aa 45-63.
Cross-Reactivity (Details) Species reactivity (tested):Mouse, rat
Purification Affinity chromatography
Immunogen Synthetic peptide from amino acids 45-63 from the unique region of the CD80

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN492862) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background The vertebrate immune system has an important self check mechanism that prevents it from destroying the host own immune system and regulatory T cells are part of this regulatory suppressive control by interacting with CD80 and CD86 (1). The cytotoxic T lymphocyte antigen 4 (CTLA4) is a protein that is expressed on regulatory T lymphocytes and is involved in restraining the immune response when needed and in tachyphylaxis and tolerance. The Fox3p-CD4+ (CD25+CD4+) regulatory T cells (Tregs) express transcription factor Foxp3 suppress aberrant immune responses as seen in autoimmune diseases and allergy (2). CD80 is a co-stimulatory ligand that interacts with CD28 and cytotoxic lymphocyte antigen 4 (CTLA4) in the regulation of T cell response. CD80 along with CD86 expression on antigen presenting cells (APCs) have the ability to augment T cell responses through interactions with CD28 (3) or inhibit T cell responses through interaction with CTLA-4 (4). The CD80/CD86 interaction with CD28/CTLA-4 occurs in the contact area between T cells and APC in parallel with T cell antigen receptor interaction with MHC-peptide complexes and LFA-1 interaction with ICAM-1 (4). The CTLA4 expressed in Fox3p positive tregs is critically required for their in vivo and in vitro suppression which is mediated at least in part by CTLA4 dependent down regulation of CD80 and CD86 in antigen presenting cells. CD80 and CD86 are cell surface markers expressed on human dendritic cells and mature B-lymphocytes and they play a critical role in stimulating T lymphocyte response by their interaction with the ligand CTLA4 and CD28. CD80 and CD86 are up-regulated during the process of dendritic cell maturation and are over expressed in variety of lymphocytic neoplasm (6). CD80 expression is selectively increases in freshly prepared spleen B cells treated with LPS for 9-12 hours and when they are exposed to X-radiation, whereas CD86 is not affected and only slight increase in CD54, major histocompatibility complex class II, CD11a or surface immunoglobulin M was observed (5). CD80/CD86 co-stimulation is important for the immune response to vesicular stomatitis virus, herpes simplex virus and influenza virus. CD80/CD86 serve a a means of adenovirus entry on human dendritic cells and the interaction of CD80/CD86 with CTLA4 is also important in long-term control of murine gammaherpesvirus-68 virus. CD80 provides a regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. CD80 is a 321 amino acid (39 kDa) protein expressed on Treg.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene ID 12519
NCBI Accession NP_033985
UniProt Q00609
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN492862) Images back to top
Application Notes Dot blot: 1: 20,000. ELISA: 1: 20,000. Western blot: 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN492862) Images back to top
Concentration 0.68 - 0.70mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-CD80 Antibody (ABIN492862)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Background publications

Torihata, Ishikawa, Okada, Tanaka, Uchida, Suguro, Kakiuchi: "Irradiation up-regulates CD80 expression through two different mechanisms in spleen B cells, B lymphoma cells, and dendritic cells." in: Immunology, Vol. 112, Issue 2, pp. 219-27, 2004

Egen, Allison: "Cytotoxic T lymphocyte antigen-4 accumulation in the immunological synapse is regulated by TCR signal strength." in: Immunity, Vol. 16, Issue 1, pp. 23-35, 2002

Maeda, Towatari, Kosugi, Saito: "Up-regulation of costimulatory/adhesion molecules by histone deacetylase inhibitors in acute myeloid leukemia cells." in: Blood, Vol. 96, Issue 12, pp. 3847-56, 2000

Thompson, Allison: "The emerging role of CTLA-4 as an immune attenuator." in: Immunity, Vol. 7, Issue 4, pp. 445-50, 1997

Linsley, Brady, Grosmaire, Aruffo, Damle, Ledbetter: "Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation." in: The Journal of experimental medicine, Vol. 173, Issue 3, pp. 721-30, 1991

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN492862) back to top
Supplier Images
 image for anti-CD80 antibody (CD80) (AA 45-63) (ABIN492862) anti-CD80 (CD80) (AA 45-63) antibody