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|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD80 antibody is conjugated to Biotin Alternatives|
Enzyme Immunoassay (EIA)
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Product Details anti-CD80 AntibodyTarget Details CD80 Application Details Handling Images
|Specificity||This antibody reatcts with Murine B-71|
|Immunogen||Highly pure (>98%) recombinant mB-71.|
Target Details CD80Product Details anti-CD80 Antibody Application Details Handling Images back to top
|Alternative Name||CD80 (CD80 Antibody Abstract)|
|Background||The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process|
Application DetailsProduct Details anti-CD80 Antibody Target Details CD80 Handling Images back to top
ELISA: To detect mB-71 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mB-71. Western Blot: To detect mB-71 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mB-71 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD80 Antibody Target Details CD80 Application Details Images back to top
|Reconstitution||Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|