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anti-Mouse (Murine) CD80 antibody for Flow Cytometry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • B71
  • BB1
  • CD8
  • Cd28l
  • CD28LG
  • CD28LG1
  • LAB7
  • Leu2
  • Ly-53
  • Ly53
  • MAL
  • MIC17
  • p32
  • TSA1
Reactivity
Mouse (Murine)
411
275
50
17
10
9
8
3
2
2
2
Host
Rat
383
112
99
73
50
17
Clonality
Monoclonal
Conjugate
This CD80 antibody is conjugated to FITC
79
77
52
26
17
15
15
12
11
11
7
7
6
6
6
6
6
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
495
207
116
115
102
66
46
44
33
22
17
16
14
7
7
7
7
5
5
4
4
2
2
2
1
1
1
1
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Catalog No. ABIN371005
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN951413 FACS Rat IgG2a Log in to see RMMP-1 1
0.30226976 ABIN1176972 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16-10A1 6
0.30226976 ABIN2659319 FACS PE-Cy7 Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2659751 BR Dep FACS CyTOF IP IHC PE/Dazzle™ 594 Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2662310 FACS IF IHC Pacific Blue Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2657845 FACS CyTOF IHC Alexa Fluor 647 Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2661133 FACS Biotin Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2660414 FACS PerCP-Cy5.5 Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2663589 FACS PE Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2665750 FACS Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2664829 FACS CyTOF Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN2664828 FACS Hamster IgG Log in to see 16-10A1 11
0.30226976 ABIN440011 FACS in vitro IP Allophycocyanin Rat IgG2a kappa Log in to see 1G10
0.30226976 ABIN440010 FACS in vitro IP PE Rat IgG2a kappa Log in to see 1G10
0.30226976 ABIN440009 FACS in vitro IP Biotin Rat IgG2a kappa Log in to see 1G10
0.30226976 ABIN440008 FACS in vitro IP FITC Rat IgG2a kappa Log in to see 1G10
0.30226976 ABIN2679439 FACS PE-Cy7 Armenian Hamster IgG Log in to see 16-10A1
0.30226976 ABIN2679441 FACS PerCP-eFluor® 710 Armenian Hamster IgG Log in to see 16-10A1
0.30226976 ABIN2679443 FACS EFluor 450 Armenian Hamster IgG Log in to see 16-10A1
0.30226976 ABIN2169166 FACS APC Rat IgG2a Log in to see 1G10

Top referenced anti-CD80 antibody for Flow Cytometry

Similar anti-CD80 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
Blocking Reagent (BR) 6 Antibodies
Depletion (Dep) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 21 Antibodies
ELISA (Capture) 4 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 220 Antibodies
Functional Studies (Func) 21 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 56 Antibodies

General

Antigen CD80 Antibodies
Reactivity Mouse (Murine)
(411), (275), (50), (17), (10), (9), (8), (3), (2), (2), (2)
Host Rat
(383), (112), (99), (73), (50), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is conjugated to FITC
(79), (77), (52), (26), (17), (15), (15), (12), (11), (11), (7), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(495), (207), (116), (115), (102), (66), (46), (44), (33), (22), (17), (16), (14), (7), (7), (7), (7), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN371005) Images
Specificity This antibody recognises CD80/B7-1 (Mr. 45-47 kDa). This Monoclonal CD80 antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Characteristics Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1
Clone 1G10
Isotype IgG2a

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN371005) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene ID 12519
UniProt Q00609
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN371005) Images back to top
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.2,7)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN371005) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD80 Antibody (ABIN371005)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Background publications

Natesan, Razi-Wolf, Reiser: "Costimulation of IL-4 production by murine B7-1 and B7-2 molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2783-91, 1996

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, 1994

Powers, Faherty, Connaughton, Biondi, Godfrey, Gault, Chen, Nabavi: "Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody." in: Cellular immunology, Vol. 153, Issue 2, pp. 298-311, 1994

Nabavi, Freeman, Gault, Godfrey, Nadler, Glimcher: "Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression." in: Nature, Vol. 360, Issue 6401, pp. 266-8, 1992

Razi-Wolf, Freeman, Galvin, Benacerraf, Nadler, Reiser: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, 1992

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN371005) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 antibody (CD80)  (FITC) (ABIN371005) anti-CD80 (CD80) antibody (FITC)