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|Application / Reactivity||Mouse (Murine)|
|Blocking Antibody (Inhibition)||2 Antibodies|
|Blocking Reagent (BR)||6 Antibodies|
|Depletion (Dep)||2 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|ELISA (Capture)||4 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||170 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||25 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||17 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||52 Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD80 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CD80 AntibodyTarget Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN371005) Images
|Specificity||This antibody recognises CD80/B7-1 (Mr. 45-47 kDa). This Monoclonal CD80 antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).|
|Cross-Reactivity (Details)||Species reactivity (tested):Mouse.|
|Characteristics||Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1|
Target Details CD80Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN371005) Images back to top
|Alternative Name||CD80 (CD80 Antibody Abstract)|
|Background||CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN371005) Images back to top
Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.2,7)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN371005) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD80 Antibody (ABIN371005)Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Natesan, Razi-Wolf, Reiser: "Costimulation of IL-4 production by murine B7-1 and B7-2 molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2783-91, 1996 (PubMed).
Hathcock, Laszlo, Pucillo et al.: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, 1994 (PubMed).
Powers, Faherty, Connaughton et al.: "Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody." in: Cellular immunology, Vol. 153, Issue 2, pp. 298-311, 1994 (PubMed).
Razi-Wolf, Freeman, Galvin et al.: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, 1992 (PubMed).
Nabavi, Freeman, Gault et al.: "Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression." in: Nature, Vol. 360, Issue 6401, pp. 266-8, 1992 (PubMed).
ImagesProduct Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN371005) back to top