|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Blocking Reagent (BR)||1 Antibodies||6 Antibodies|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||5 Antibodies||21 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||30 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Western Blotting (WB)||10 Antibodies|
|Blocking Antibody (Inhibition)||2 Antibodies|
|Depletion (Dep)||2 Antibodies|
|Epitope||AA 45-63 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CD80 antibody is un-conjugated Alternatives|
Dot Blot (DB), Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CD80 AntibodyTarget Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN492862) Images
|Sequence||YDH FID CFI EYG DAH VS|
|Specificity||This antibody detects CD80 at aa 45-63.|
|Cross-Reactivity (Details)||Species reactivity (tested):Mouse, rat|
|Immunogen||Synthetic peptide from amino acids 45-63 from the unique region of the CD80|
Target Details CD80Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN492862) Images back to top
|Alternative Name||CD80 (CD80 Antibody Abstract)|
|Background||The vertebrate immune system has an important self check mechanism that prevents it from destroying the host own immune system and regulatory T cells are part of this regulatory suppressive control by interacting with CD80 and CD86 (1). The cytotoxic T lymphocyte antigen 4 (CTLA4) is a protein that is expressed on regulatory T lymphocytes and is involved in restraining the immune response when needed and in tachyphylaxis and tolerance. The Fox3p-CD4+ (CD25+CD4+) regulatory T cells (Tregs) express transcription factor Foxp3 suppress aberrant immune responses as seen in autoimmune diseases and allergy (2). CD80 is a co-stimulatory ligand that interacts with CD28 and cytotoxic lymphocyte antigen 4 (CTLA4) in the regulation of T cell response. CD80 along with CD86 expression on antigen presenting cells (APCs) have the ability to augment T cell responses through interactions with CD28 (3) or inhibit T cell responses through interaction with CTLA-4 (4). The CD80/CD86 interaction with CD28/CTLA-4 occurs in the contact area between T cells and APC in parallel with T cell antigen receptor interaction with MHC-peptide complexes and LFA-1 interaction with ICAM-1 (4). The CTLA4 expressed in Fox3p positive tregs is critically required for their in vivo and in vitro suppression which is mediated at least in part by CTLA4 dependent down regulation of CD80 and CD86 in antigen presenting cells. CD80 and CD86 are cell surface markers expressed on human dendritic cells and mature B-lymphocytes and they play a critical role in stimulating T lymphocyte response by their interaction with the ligand CTLA4 and CD28. CD80 and CD86 are up-regulated during the process of dendritic cell maturation and are over expressed in variety of lymphocytic neoplasm (6). CD80 expression is selectively increases in freshly prepared spleen B cells treated with LPS for 9-12 hours and when they are exposed to X-radiation, whereas CD86 is not affected and only slight increase in CD54, major histocompatibility complex class II, CD11a or surface immunoglobulin M was observed (5). CD80/CD86 co-stimulation is important for the immune response to vesicular stomatitis virus, herpes simplex virus and influenza virus. CD80/CD86 serve a a means of adenovirus entry on human dendritic cells and the interaction of CD80/CD86 with CTLA4 is also important in long-term control of murine gammaherpesvirus-68 virus. CD80 provides a regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. CD80 is a 321 amino acid (39 kDa) protein expressed on Treg.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN492862) Images back to top
Dot blot: 1: 20,000. ELISA: 1: 20,000. Western blot: 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN492862) Images back to top
|Concentration||0.68 - 0.70mg/mL|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store (in aliquots) at -20 °C.|
References for anti-CD80 Antibody (ABIN492862)Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Torihata, Ishikawa, Okada et al.: "Irradiation up-regulates CD80 expression through two different mechanisms in spleen B cells, B lymphoma cells, and dendritic cells." in: Immunology, Vol. 112, Issue 2, pp. 219-27, 2004 (PubMed).
Egen, Allison: "Cytotoxic T lymphocyte antigen-4 accumulation in the immunological synapse is regulated by TCR signal strength." in: Immunity, Vol. 16, Issue 1, pp. 23-35, 2002 (PubMed).
Maeda, Towatari, Kosugi et al.: "Up-regulation of costimulatory/adhesion molecules by histone deacetylase inhibitors in acute myeloid leukemia cells." in: Blood, Vol. 96, Issue 12, pp. 3847-56, 2000 (PubMed).
Thompson, Allison: "The emerging role of CTLA-4 as an immune attenuator." in: Immunity, Vol. 7, Issue 4, pp. 445-50, 1997 (PubMed).
Linsley, Brady, Grosmaire et al.: "Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation." in: The Journal of experimental medicine, Vol. 173, Issue 3, pp. 721-30, 1991 (PubMed).
ImagesProduct Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN492862) back to top