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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||30 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Western Blotting (WB)||10 Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD80 antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CD80 AntibodyTarget Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN2689408) Images
The 3H5 antibody reacts with CD80 (B7-1), a member of the Ig superfamily of transmembrane proteins. CD80, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD80 is predominantly expressed on antigen-presenting cells. It can be induced on splenic B cells by in vitro polyclonal activation such as LPS treatment. 3H5 mAb is reported to block the costimulatory function of rat CD80 and to immunoprecipitate CD80 from Lewis-S1 cell lysates.
BD Pharmingen™ Purified Mouse Anti-Rat CD80 - Purified - Clone 3H5 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Rat HTLV-1-transformed T-cell line Lewis-S1|
Target Details CD80Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN2689408) Images back to top
|Alternative Name||CD80 (CD80 Antibody Abstract)|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN2689408) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN2689408) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-CD80 Antibody (ABIN2689408)Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
|Product cited in:||
Damoiseaux, Yagita, Okumura et al.: "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, 1998 (PubMed).
Maeda, Sato, Azuma et al.: "Characterization of rat CD80 and CD86 by molecular cloning and mAb." in: International immunology, Vol. 9, Issue 7, pp. 993-1000, 1997 (PubMed).
Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995 (PubMed).