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|Antigen||CD80 ELISA Kits|
|Reactivity||Mouse (Murine) Alternatives|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||62.5-4000 pg/mL|
|Minimum Detection Limit||62.5 pg/mL|
|3 references available|
|Supplier||Log in to see|
Product Details CD80 ELISA KitTarget details Application Details Handling ProductDetails: References for CD80 Kit (ABIN921092) Images
|Purpose||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse B7-1/CD80|
|Sample Type||Cell Culture Supernatant, Serum|
Expression system for standard: NSO
Immunogen sequence: D37-K245
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: NSO
Immunogen sequence: D37-K245
Target detailsProduct Details CD80 ELISA Kit Application Details Handling ProductDetails: References for CD80 Kit (ABIN921092) Images back to top
|Alternative Name||CD80 (CD80 ELISA Kit Abstract)|
Protein Function: Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Background: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86(601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1, D37-K245, which is composed of two single chains acids with the dipolymer.
Synonyms: T-lymphocyte activation antigen CD80,Activation B7-1 antigen,B7,CD80,Cd80,B7,
Full Gene Name: T-lymphocyte activation antigen CD80Cellular Localisation: Membrane, Single-pass type I membrane protein.
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process|
Application DetailsProduct Details CD80 ELISA Kit Target details Handling ProductDetails: References for CD80 Kit (ABIN921092) Images back to top
|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Tissue Specificity: Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||mouse CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD80 has been precoated onto 96-well plates. Standards(NSO, D37-K245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD80 standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details CD80 ELISA Kit Target details Application Details ProductDetails: References for CD80 Kit (ABIN921092) Images back to top
|Handling Advice||Avoid multiple freeze-thaw cycles.|
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
ProductDetails: References for CD80 Kit (ABIN921092)Product Details CD80 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Du, Dong, Zhao, Fu, Wang, Chen, Ou, Li, Sun, Tang, Song: "Immunostimulatory and anti-neoplasm effects of a novel palindrome CpG oligodeoxynucleotide in mice." in: Acta pharmacologica Sinica, Vol. 33, Issue 8, pp. 1047-54, 2012
Reeves, Patch, Sharpe, Borriello, Freeman, Edelhoff, Disteche: "The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 8, Issue 8, pp. 581-2, 1997
Selvakumar, Mohanraj, Eddy, Shows, White, Dupont: "Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7." in: Immunogenetics, Vol. 36, Issue 3, pp. 175-81, 1992
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