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Rat (Rattus) CD80 ELISA Kit

Recommended CD80 Product (supplied by: Log in to see )

Antigen
CD80 ELISA Kits
  • B7
  • B7-1
  • B7.1
  • B71
  • BB1
  • CD8
  • Cd28l
  • CD28LG
  • CD28LG1
  • LAB7
  • Leu2
  • Ly-53
  • Ly53
  • MAL
  • MIC17
  • p32
  • TSA1
Epitope
AA 39-248
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
12
9
3
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1889314
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN2708631 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012121415 ABIN2683759 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012121415 ABIN2748018 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids Log in to see

Top referenced CD80 ELISA Kit for ELISA

Similar CD80 ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 4 ELISA Kits

General

Antigen CD80 ELISA Kits
Epitope AA 39-248
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(12), (9), (3)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CD80 ELISA Kit

Target details Application Details Handling References for CD80 Kit (ABIN1889314) Images
Purpose For quantitative detection of rat CD80 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant rat CD80
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- rat CD80 antibod - 1
  • Lyophilized recombinant rat CD80 standar - 10ng/tubex2
  • Biotinylated anti- rat CD80 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: I39-Q248

Target details

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Antigen
Alternative Name CD80 (CD80 ELISA Kit Abstract)
Background The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Gene ID 25408
UniProt O35187
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, I39-Q248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD80 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CD80 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.18, Standard deviation: 0.06, CV(%): 5.1
  • Sample 2: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.159, CV(%): 6.8
  • Sample 3: n=16, Mean(ng/ml): 3.12, Standard deviation: 0.175, CV(%): 5.6,
  • Sample 1: n=24, Mean(ng/ml): 1.19, Standard deviation: 0.069, CV(%): 5.8
  • Sample 2: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.182, CV(%): 7.4
  • Sample 3: n=24, Mean(ng/ml): 3.35, Standard deviation: 0.224, CV(%): 6.7
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CD80 Kit (ABIN1889314)

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Background publications

Reeves, Patch, Sharpe, Borriello, Freeman, Edelhoff, Disteche: "The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 8, Issue 8, pp. 581-2, 1997

Selvakumar, Mohanraj, Eddy, Shows, White, Dupont: "Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7." in: Immunogenetics, Vol. 36, Issue 3, pp. 175-81, 1992

Images

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Supplier Images
ELISA image for CD80 ELISA Kit (CD80) (ABIN1889314) Rat CD80/B7-1 PicoKine ELISA Kit standard curve