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anti-Human CREB1 antibody for Immunoprecipitation

Recommended CREB1 Antibody (supplied by: Log in to see )

Antigen
CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • CREB
  • creb
  • Creb-1
  • creb1
  • Creb1
  • CREB1
  • zgc:55598
Epitope
pSer133
111
36
34
33
28
21
17
17
16
13
10
7
7
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
492
251
233
27
25
19
18
15
14
9
9
9
2
2
1
1
1
Host
Rabbit
424
74
10
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
17
15
14
7
6
6
6
5
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
393
224
187
73
65
46
36
34
26
10
9
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN116949
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN116948 EMSA EIA IP WB Rabbit N-Term Log in to see Polyclonal 1
0.00089556724 ABIN151873 IP WB Rabbit pSer121 Log in to see Polyclonal 5
0.00089556724 ABIN189267 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser133 Log in to see E113
0.00089556724 ABIN189266 GS ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see E306
0.00089556724 ABIN2705937 IP IHC WB Rabbit pSer133 Log in to see Polyclonal
0.00089556724 ABIN318001 IHC (p) IP WB Rabbit pSer133 Log in to see Polyclonal
0.00089556724 ABIN232806 IP WB Rabbit IgG AA 5-45 Log in to see Polyclonal
0.00089556724 ABIN97863 IP IHC ELISA WB Rabbit IgG AA 122-147, pSer133 Log in to see Polyclonal
0.00089556724 ABIN211132 IP WB Rabbit IgG AA 5-24 Log in to see Polyclonal
0.00089556724 ABIN1871996 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN261497 ICC IF IHC (p) IP IHC ELISA WB Mouse IgG1 Log in to see LB9
0.00089556724 ABIN257735 ICC IF IHC (p) IP IHC PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal
0.00089556724 ABIN2163957 IP IHC ELISA WB Rabbit IgG Ser133 Log in to see Polyclonal
0.00089556724 ABIN200676 IP ELISA WB Rabbit pSer133 Log in to see Polyclonal
0.00089556724 ABIN227649 ICC IHC (p) IP ELISA WB Rabbit IgG pSer133 Log in to see Polyclonal
0.00089556724 ABIN353293 FACS IHC (fro) IF IHC (p) IP WB Rabbit IgG Log in to see
0.00089556724 ABIN2893203 IP IHC WB Rabbit pSer133 Log in to see Polyclonal
0.00089556724 ABIN2883668 IP IHC ELISA WB Rabbit IgG pSer133 Log in to see Polyclonal
0.00089556724 ABIN3181974 ELISA IHC IP WB Rabbit IgG pSer133 Log in to see Polyclonal
0.00089556724 ABIN2240719 IP WB Rabbit IgG pSer133 Log in to see 10E17

Top referenced anti-CREB1 antibody for Immunoprecipitation

Similar anti-CREB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 73 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 36 Antibodies
Immunohistochemistry (IHC) 122 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 20 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 168 Antibodies

General

Antigen CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
Epitope pSer133
(111), (36), (34), (33), (28), (21), (17), (17), (16), (13), (10), (7), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(492), (251), (233), (27), (25), (19), (18), (15), (14), (9), (9), (9), (2), (2), (1), (1), (1)
Host Rabbit
(424), (74), (10), (1)
Clonality
Polyclonal
Conjugate This CREB1 antibody is un-conjugated
(17), (15), (14), (7), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(393), (224), (187), (73), (65), (46), (36), (34), (26), (10), (9), (4), (3), (2), (2), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CREB1 Antibody

Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN116949) Images
Sequence Q-K-R-R-E-I-L-S-R-R-P-pS-Y-R-K-I-L-N-D-L-S-S-D-A-P correspond ing to aa 122-147
Specificity This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.
Purification Affinity Purified
Immunogen CREB phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Target Details CREB1

Product Details anti-CREB1 Antibody Application Details Handling References for anti-CREB1 Antibody (ABIN116949) Images back to top
Antigen
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 1385
NCBI Accession NP_004370
UniProt P16220
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CREB1 Antibody Target Details CREB1 Handling References for anti-CREB1 Antibody (ABIN116949) Images back to top
Application Notes Western Blot: 1: 1,000. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details References for anti-CREB1 Antibody (ABIN116949) Images back to top
Format Liquid
Concentration 0.064 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.1 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CREB1 Antibody (ABIN116949)

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Background publications

Arthur, Cohen: "MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells." in: FEBS letters, Vol. 482, Issue 1-2, pp. 44-8, 2000

Kim, Lu, Quinn: "Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 21, pp. 11292-6, 2000

Moreno, Beresford, Louis-Plence, Morris, Boss: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999

Kwok, Laurance, Lundblad, Goldman, Shih, Connor, Marriott, Goodman: "Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP." in: Nature, Vol. 380, Issue 6575, pp. 642-6, 1996

Barton, Muthusamy, Chanyangam, Fischer, Clendenin, Leiden: "Defective thymocyte proliferation and IL-2 production in transgenic mice expressing a dominant-negative form of CREB." in: Nature, Vol. 379, Issue 6560, pp. 81-5, 1996

Lee, Yun, Hoeffler, Habener: "Cyclic-AMP-responsive transcriptional activation of CREB-327 involves interdependent phosphorylated subdomains." in: The EMBO journal, Vol. 9, Issue 13, pp. 4455-65, 1991

Dwarki, Montminy, Verma: "Both the basic region and the 'leucine zipper' domain of the cyclic AMP response element binding (CREB) protein are essential for transcriptional activation." in: The EMBO journal, Vol. 9, Issue 1, pp. 225-32, 1990

Images

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN116949) back to top
Supplier Images
 image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133) (ABIN116949) Anti-CREB pS133 was used to detect phosphorylated CREB by immunoblot. Recombinant His...
 image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133) (ABIN116949) Anti-CREB pS133 was used to detect phosphorylated CREB by immunoblot. Recombinant His...