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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||1 Antibodies||1 Antibodies|
|ELISA||71 Antibodies||72 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies|
|Immunofluorescence (IF)||27 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||39 Antibodies|
|Immunohistochemistry (IHC)||113 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies|
|Immunoprecipitation (IP)||19 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Western Blotting (WB)||158 Antibodies|
|Antigen||CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CREB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-CREB1 AntibodyTarget Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN116949) Images
|Sequence||Q-K-R-R-E-I-L-S-R-R-P-pS-Y-R-K-I-L-N-D-L-S-S-D-A-P correspond ing to aa 122-147|
|Specificity||This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.|
|Immunogen||CREB phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
Target Details CREB1Product Details anti-CREB1 Antibody Application Details Handling References for anti-CREB1 Antibody (ABIN116949) Images back to top
|Alternative Name||CREB1 (CREB1 Antibody Abstract)|
|Background||The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1|
|Pathways||TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CREB1 Antibody Target Details CREB1 Handling References for anti-CREB1 Antibody (ABIN116949) Images back to top
Western Blot: 1: 1,000. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details References for anti-CREB1 Antibody (ABIN116949) Images back to top
|Concentration||0.064 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.1 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CREB1 Antibody (ABIN116949)Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Kim, Lu, Quinn: "Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 21, pp. 11292-6, 2000 (PubMed).
Arthur, Cohen: "MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells." in: FEBS letters, Vol. 482, Issue 1-2, pp. 44-8, 2000 (PubMed).
Moreno, Beresford, Louis-Plence et al.: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999 (PubMed).
Kwok, Laurance, Lundblad et al.: "Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP." in: Nature, Vol. 380, Issue 6575, pp. 642-6, 1996 (PubMed).
Barton, Muthusamy, Chanyangam et al.: "Defective thymocyte proliferation and IL-2 production in transgenic mice expressing a dominant-negative form of CREB." in: Nature, Vol. 379, Issue 6560, pp. 81-5, 1996 (PubMed).
Lee, Yun, Hoeffler et al.: "Cyclic-AMP-responsive transcriptional activation of CREB-327 involves interdependent phosphorylated subdomains." in: The EMBO journal, Vol. 9, Issue 13, pp. 4455-65, 1991 (PubMed).
Dwarki, Montminy, Verma: "Both the basic region and the 'leucine zipper' domain of the cyclic AMP response element binding (CREB) protein are essential for transcriptional activation." in: The EMBO journal, Vol. 9, Issue 1, pp. 225-32, 1990 (PubMed).
ImagesProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN116949) back to top