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anti-Human CrkL antibody for RNA Interference

Recommended CrkL Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
  • 1110025F07Rik
  • AA589403
  • AI325100
  • CRKL
  • Crkol
  • wu:fa55g05
  • wu:fj60e01
  • zgc:63469
Epitope
AA 204-303, partial
39
18
16
15
14
13
12
12
9
7
5
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human
137
80
57
2
1
1
Host
Mouse
106
26
24
1
Clonality
Monoclonal
Conjugate
This CrkL antibody is un-conjugated
7
7
7
6
5
5
3
3
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Proximity Ligation Assay (PLA), RNA Interference (RNAi), ELISA, Western Blotting (WB)
127
70
30
21
18
16
9
3
3
3
2
1
1
1
Supplier
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Catalog No. ABIN514606
$ 430.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008752735 ABIN1827663 IF RNAi ELISA WB Mouse IgG2b, kappa Log in to see 4B5

Top referenced anti-CrkL antibody for RNA Interference

Similar anti-CrkL Antibodies

Application / Reactivity Human
ELISA 57 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 21 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 110 Antibodies

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
Epitope AA 204-303, partial
(39), (18), (16), (15), (14), (13), (12), (12), (9), (7), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(137), (80), (57), (2), (1), (1)
Host Mouse
(106), (26), (24), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CrkL antibody is un-conjugated
(7), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Proximity Ligation Assay (PLA), RNA Interference (RNAi), ELISA, Western Blotting (WB)
(127), (70), (30), (21), (18), (16), (9), (3), (3), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CrkL Antibody

Target Details CrkL Application Details Handling References for anti-CrkL Antibody (ABIN514606) Images
Cross-Reactivity Human
Immunogen CRKL (NP_005198, 204 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: AHAYAQPQTT TPLPAVSGSP GAAITPLPST QNGPVFAKAI QKRVPCAYDK TALALEVGDI VKVTRMNING QWEGEVNGRK GLFPFTHVKI FDPQNPDENE
Clone 4B5
Isotype IgG2b kappa

Target Details CrkL

Product Details anti-CrkL Antibody Application Details Handling References for anti-CrkL Antibody (ABIN514606) Images back to top
Antigen
Alternative Name CRKL (CRKL Antibody Abstract)
Background Full Gene Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Synonyms:
Gene ID 1399
NCBI Accession NP_005198, NM_005207
Pathways TCR Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CrkL Antibody Target Details CrkL Handling References for anti-CrkL Antibody (ABIN514606) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Product Quality tested by: Antibody Reactive Against Recombinant Protein.

Restrictions For Research Use only

Handling

Product Details anti-CrkL Antibody Target Details CrkL Application Details References for anti-CrkL Antibody (ABIN514606) Images back to top
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CrkL Antibody (ABIN514606)

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling Images back to top
Product cited in:

Liu, Chen, Chau, Jan, Chen, Hsu, Lin, Juang, Lu, Cheng, Chen, Chang, Ting, Kao, Hsiao, Huang: "Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 5, pp. 1335-49, 2013

Segovis, Schoon, Dick, Nacusi, Leibson, Billadeau: "PI3K links NKG2D signaling to a CrkL pathway involved in natural killer cell adhesion, polarity, and granule secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 11, pp. 6933-42, 2009

Images

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling References for anti-CrkL Antibody (ABIN514606) back to top
Supplier Images
ELISA image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Detection limit for recombinant GST tagged CRKL is approximately 0.3ng/ml as a captur...
Western Blotting (WB) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL V...
Western Blotting (WB) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL monocl...
Proximity Ligation Assay (PLA) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Proximity Ligation Analysis of protein-protein interactions between PTK2 and CRKL. Hu...
Proximity Ligation Assay (PLA) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Proximity Ligation Analysis of protein-protein interactions between GAB1 and CRKL. He...
Proximity Ligation Assay (PLA) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Proximity Ligation Analysis of protein-protein interactions between HCK and CRKL. Mah...
Immunofluorescence (IF) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Immunofluorescence of monoclonal antibody to CRKL on HeLa cell. [antibody concentrati...
 image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (AA 204-303) (ABIN514606) Western Blot detection against Immunogen (36.74 KDa) .