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|Application / Reactivity||Human|
|ELISA||3 ELISA Kits|
|Antigen||Drebrin-Like (DBNL) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||1.56-100 ng/mL|
|Minimum Detection Limit||1.56 ng/mL|
|Supplier||Log in to see|
Product Details DBNL ELISA KitTarget details Application Details Handling Images
|Purpose||ELISA Kit for Drebrin Like Protein (DBNL)|
|Sample Type||Cell Lysate, Tissue Homogenate, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Drebrin Like Protein (DBNL).
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|Alternative Name||Drebrin Like Protein (DBNL ELISA Kit Abstract)|
Application DetailsProduct Details DBNL ELISA Kit Target details Handling Images back to top
|Assay Time||3 h|
|Protocol||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Drebrin Like Protein (DBNL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Drebrin Like Protein (DBNL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Drebrin Like Protein (DBNL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Drebrin Like Protein (DBNL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Drebrin Like Protein (DBNL) were tested 20 times on one plate, respectively.
|Restrictions||For Research Use only|
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.