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anti-Human ITK antibody for Enzyme Immunoassay

Recommended ITK Antibody (supplied by: Log in to see )

Antigen
IL2-Inducible T-Cell Kinase (ITK) Antibodies
  • Emt
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • Tcsk
  • Tsk
Epitope
Middle Region
22
20
9
9
9
8
8
7
5
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
102
24
17
3
1
1
1
1
Host
Rabbit
58
29
25
Clonality
Polyclonal
Conjugate
This ITK antibody is un-conjugated
7
6
6
6
6
6
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
94
73
7
6
6
6
6
4
2
1
1
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Catalog No. ABIN359985
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN1109424 EIA IF WB Mouse IgG1 Log in to see 5G6 1
0.0008870071 ABIN1109423 EIA WB Mouse IgG1 Log in to see 5G12C4 3

Top referenced anti-ITK antibody for Enzyme Immunoassay

Similar anti-ITK Antibodies

Application / Reactivity Human
Dot Blot (DB) 1 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 90 Antibodies

General

Antigen IL2-Inducible T-Cell Kinase (ITK) Antibodies
Epitope Middle Region
(22), (20), (9), (9), (9), (8), (8), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(102), (24), (17), (3), (1), (1), (1), (1)
Host Rabbit
(58), (29), (25)
Clonality
Polyclonal
Conjugate This ITK antibody is un-conjugated
(7), (6), (6), (6), (6), (6)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(94), (73), (7), (6), (6), (6), (6), (4), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-ITK Antibody

Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN359985) Images
Specificity This antibody reacts to ITK.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ITK.
Isotype Ig

Target Details ITK

Product Details anti-ITK Antibody Application Details Handling References for anti-ITK Antibody (ABIN359985) Images back to top
Antigen
Alternative Name Tyrosine-Protein Kinase ITK/TSK (ITK Antibody Abstract)
Background ITK is an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.Synonyms: EMT, Kinase EMT, LYK, T-cell-specific kinase, Tyrosine-protein kinase Lyk
Gene ID 3702
NCBI Accession NP_005537
UniProt Q08881
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ITK Antibody Target Details ITK Handling References for anti-ITK Antibody (ABIN359985) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ITK Antibody Target Details ITK Application Details References for anti-ITK Antibody (ABIN359985) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ITK Antibody (ABIN359985)

Product Details anti-ITK Antibody Target Details ITK Application Details Handling Images back to top
Background publications

Brazin, Mallis, Fulton, Andreotti: "Regulation of the tyrosine kinase Itk by the peptidyl-prolyl isomerase cyclophilin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 1899-904, 2002

Hawkins, Marcy: "Characterization of Itk tyrosine kinase: contribution of noncatalytic domains to enzymatic activity." in: Protein expression and purification, Vol. 22, Issue 2, pp. 211-9, 2001

Janis, Siliciano, Isaac, Griffin, Hawkins, Kozak, Desiderio: "Mapping of the gene for the tyrosine kinase Itk to a region of conserved synteny between mouse chromosome 11 and human chromosome 5q." in: Genomics, Vol. 23, Issue 1, pp. 269-71, 1995

Gibson, Leung, Squire, Hill, Arima, Goss, Hogg, Mills: "Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q." in: Blood, Vol. 82, Issue 5, pp. 1561-72, 1993

Tanaka, Asao, Ohtani, Nakamura, Sugamura: "A novel human tyrosine kinase gene inducible in T cells by interleukin 2." in: FEBS letters, Vol. 324, Issue 1, pp. 1-5, 1993

Images

Product Details anti-ITK Antibody Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN359985) back to top
Supplier Images
 image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (Middle Region) (ABIN359985) anti-IL2-Inducible T-Cell Kinase (ITK) (Middle Region) antibody