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|Antigen||IL2-Inducible T-Cell Kinase (ITK) Antibodies|
|Epitope||Middle Region Alternatives|
|Conjugate||This ITK antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
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Product Details anti-ITK AntibodyTarget Details ITK Application Details Handling References for anti-ITK antibody (ABIN359985) Images
|Specificity||This antibody reacts to ITK.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ITK.|
Target Details ITKProduct Details anti-ITK Antibody Application Details Handling References for anti-ITK antibody (ABIN359985) Images back to top
|Alternative Name||Tyrosine-Protein Kinase ITK/TSK (ITK Antibody Abstract)|
|Background||ITK is an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.Synonyms: EMT, Kinase EMT, LYK, T-cell-specific kinase, Tyrosine-protein kinase Lyk|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-ITK Antibody Target Details ITK Handling References for anti-ITK antibody (ABIN359985) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ITK Antibody Target Details ITK Application Details References for anti-ITK antibody (ABIN359985) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-ITK antibody (ABIN359985)Product Details anti-ITK Antibody Target Details ITK Application Details Handling Images back to top
Brazin, Mallis, Fulton, Andreotti: "Regulation of the tyrosine kinase Itk by the peptidyl-prolyl isomerase cyclophilin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 1899-904, 2002
Hawkins, Marcy: "Characterization of Itk tyrosine kinase: contribution of noncatalytic domains to enzymatic activity." in: Protein expression and purification, Vol. 22, Issue 2, pp. 211-9, 2001
Janis, Siliciano, Isaac, Griffin, Hawkins, Kozak, Desiderio: "Mapping of the gene for the tyrosine kinase Itk to a region of conserved synteny between mouse chromosome 11 and human chromosome 5q." in: Genomics, Vol. 23, Issue 1, pp. 269-71, 1995
Gibson, Leung, Squire, Hill, Arima, Goss, Hogg, Mills: "Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q." in: Blood, Vol. 82, Issue 5, pp. 1561-72, 1993
Tanaka, Asao, Ohtani, Nakamura, Sugamura: "A novel human tyrosine kinase gene inducible in T cells by interleukin 2." in: FEBS letters, Vol. 324, Issue 1, pp. 1-5, 1993
ImagesProduct Details anti-ITK Antibody Target Details ITK Application Details Handling References for anti-ITK antibody (ABIN359985) back to top