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anti-Human ITK antibody for Immunocytochemistry

Recommended ITK Antibody (supplied by: Log in to see )

Antigen
IL2-Inducible T-Cell Kinase (ITK) Antibodies
  • Emt
  • Tcsk
  • Tsk
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • IL2 inducible T cell kinase
  • IL2-inducible T-cell kinase
  • Itk
  • ITK
Reactivity
Human
103
24
18
3
2
1
1
1
Host
Mouse
59
31
24
Clonality
Monoclonal
Conjugate
This ITK antibody is un-conjugated
7
6
6
6
6
6
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
98
75
8
6
6
6
6
5
3
2
1
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Catalog No. ABIN969222
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2855946 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1498917 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 5G6
1 ABIN441040 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN4327780 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 5G6
1 ABIN4880343 ELISA FACS ICC WB Mouse IgG1 Log in to see 5G6

Top referenced anti-ITK antibody for Immunocytochemistry

  • Human Monoclonal ITK Primary Antibody for ICC, FACS - ABIN969222 (2 Publications): Huck, Feyen, Niehues, Rüschendorf, Hübner, Laws, Telieps, Knapp, Wacker, Meindl, Jumaa, Borkhardt: Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation. in The Journal of clinical investigation 2009 (PubMed)

Similar anti-ITK Antibodies

Application / Reactivity Human
ELISA 67 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 88 Antibodies

General

Antigen IL2-Inducible T-Cell Kinase (ITK) Antibodies
Reactivity Human
(103), (24), (18), (3), (2), (1), (1), (1)
Host Mouse
(59), (31), (24)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ITK antibody is un-conjugated
(7), (6), (6), (6), (6), (6)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(98), (75), (8), (6), (6), (6), (6), (5), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-ITK Antibody

Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN969222) Images
Purification Ascites
Immunogen Purified recombinant fragment of human ITK expressed in E. coli.
Clone 5G6
Isotype IgG1

Target Details ITK

Product Details anti-ITK Antibody Application Details Handling References for anti-ITK Antibody (ABIN969222) Images back to top
Antigen
Alternative Name ITK (ITK Antibody Abstract)
Background This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
Molecular Weight 72 kDa
Gene ID 3702
HGNC 3702
Research Area Signaling, Tyrosine Kinases
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ITK Antibody Target Details ITK Handling References for anti-ITK Antibody (ABIN969222) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-ITK Antibody Target Details ITK Application Details References for anti-ITK Antibody (ABIN969222) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-ITK Antibody (ABIN969222)

Product Details anti-ITK Antibody Target Details ITK Application Details Handling Images back to top
Product cited in:

Huck, Feyen, Niehues, Rüschendorf, Hübner, Laws, Telieps, Knapp, Wacker, Meindl, Jumaa, Borkhardt: "Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation." in: The Journal of clinical investigation, Vol. 119, Issue 5, pp. 1350-8, 2009

Kogina, Shoda, Yamaguchi, Tsuno, Takahashi, Fujio, Yamamoto: "Tacrolimus differentially regulates the proliferation of conventional and regulatory CD4(+) T cells." in: Molecules and cells, Vol. 28, Issue 2, pp. 125-30, 2009

Images

Product Details anti-ITK Antibody Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN969222) back to top
Supplier Images
ELISA image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (ABIN969222) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (ABIN969222) Figure 2: Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and neg...
Immunocytochemistry (ICC) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (ABIN969222) Figure 3: Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green) . B...
Western Blotting (WB) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (ABIN969222) Figure 4: Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
Western Blotting (WB) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (ABIN969222) Figure 5: Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombina...