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|Application / Reactivity||Human|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||88 Antibodies|
|Antigen||IL2-Inducible T-Cell Kinase (ITK) Antibodies|
|Conjugate||This ITK antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-ITK AntibodyTarget Details ITK Application Details Handling Images
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Immunogen||A synthetic peptide corresponding to residues in the N-term of human Emtk/Itk/Tsk was used as immunogen.|
Target Details ITKProduct Details anti-ITK Antibody Application Details Handling Images back to top
|Alternative Name||Tyrosine-protein kinase ITK/TSK (ITK Antibody Abstract)|
Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressedin T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK),a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRChomology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well theamino-terminal myristylation signal and the negative autoregulatory phosphorylation sitepresent in all members of the Src family of PTKs (3). Additionally, the Src tyrosinekinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4). Alternate Names: anti-EMT antibody, anti-Homolog of mouse T cell itk/tsk antibody, anti-IL2 inducible Tcell kinase antibody, anti-Kinase EMT antibody, anti-LYK antibody, anti-MGC126257antibody, anti-MGC126258 antibody, anti-PSCTK 2 antibody, anti-PSCTK2 antibody,anti-T cell specific kinase antibody, anti-TSK antibody, anti-Tyrosine protein kinaseantibody, anti-Tyrosine protein kinase ITK/TSK antibody, anti-Tyrosine protein kinaseLyk antibody.
Gene Symbol: ITK
|Gene ID||3702, 16428, 363577|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-ITK Antibody Target Details ITK Handling Images back to top
|Application Notes||Recommended dilutions: Flow Cytometry 1:80, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:50, Western Blot 1:500-5000|
Protocol specific for ITK Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-ITK Antibody Target Details ITK Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-ITK Antibody Target Details ITK Application Details Handling back to top