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anti-Human LAT antibody for Immunoprecipitation

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
Epitope
Cytoplasmic Domain
28
17
15
11
9
7
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
208
84
75
1
1
1
1
Host
Mouse
131
72
13
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
8
8
6
6
6
6
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
156
68
45
33
28
27
19
9
6
2
2
2
2
2
1
1
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Catalog No. ABIN302069
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008892276 ABIN120717 FACS IP WB Mouse IgG1 Log in to see LAT-01
0.0008892276 ABIN614546 IHC (p) IP WB Mouse IgG1 Log in to see LAT-10-17 (10-17)
0.0008892276 ABIN293627 IHC (p) IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT1111
0.0008892276 ABIN2470864 IP IHC WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17)
0.0008892276 ABIN2470080 IP IHC WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT1111
0.0008892276 ABIN573989 IHC (p) IP WB Mouse IgG1, kappa Log in to see LAT-10-17 (10-17)
0.0008892276 ABIN207036 FACS IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0008892276 ABIN1821202 IP WB Mouse IgG1, kappa AA 31-233 Log in to see
0.0008892276 ABIN1724041 IHC (p) IP WB Mouse IgG1 Log in to see LAT1111
0.0008892276 ABIN1724096 IHC (p) IP WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17)
0.0008892276 ABIN335637 IP WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17)
0.0008892276 ABIN233299 IP WB Rabbit IgG pTyr191 Log in to see Polyclonal
0.0008892276 ABIN233300 IP WB Rabbit IgG pTyr171 Log in to see Polyclonal
0.0008892276 ABIN269134 FACS IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0008892276 ABIN2297617 IP WB Mouse IgG AA 31-233 Log in to see 1-T-2
0.0008892276 ABIN3200681 FACS IP WB Mouse IgG1 Cytoplasmic Domain Log in to see LAT-01
0.0008892276 ABIN327831 IP WB Mouse IgG1 Log in to see
0.0008892276 ABIN2297600 IP IHC WB Mouse IgG1 kappa Log in to see LAT-10-17 (10-17)
0.0008892276 ABIN2297620 IP WB Rabbit IgG AA 31-233 Log in to see Polyclonal
0.0008892276 ABIN2297635 IP IHC WB Mouse IgG1 Log in to see LAT1111

Top referenced anti-LAT antibody for Immunoprecipitation

Similar anti-LAT Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 25 Antibodies
Intracellular Staining (ICS) 6 Antibodies
Western Blotting (WB) 151 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope Cytoplasmic Domain
(28), (17), (15), (11), (9), (7), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(208), (84), (75), (1), (1), (1), (1)
Host Mouse
(131), (72), (13), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(8), (8), (6), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(156), (68), (45), (33), (28), (27), (19), (9), (6), (2), (2), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN302069) Images
Specificity The antibody LAT-01 reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
No Cross-Reactivity Mouse (Murine)
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.
Clone LAT-01
Isotype IgG1

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN302069) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN302069) Images back to top
Application Notes Immunoprecipitation:
Application note: Tyrosine-phosphorylated form of LAT is not optimally recognized.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN302069) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-LAT Antibody (ABIN302069)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Product cited in:

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, 2005

Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN302069) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-LAT antibody (Linker For Activation of T Cells) (Cytoplasmic Domain) (ABIN302069) Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-...