LAT antibody (C-Term)

Catalog No. ABIN185255

Product Details anti-LAT Antibody

Target: LAT (Linker For Activation of T Cells (LAT))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: LAT1

Sequence: GAPDYENLQE LN

Specificity: This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GAPDYENLQELN, from the C Terminus of the protein sequence according to NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 27.9 kDa according to NP_055202.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT (LAT Products)

Synonyms: LAT antibody, lat antibody, LAT1 antibody, pp36 antibody, linker for activation of T-cells antibody, linker for activation of T cells antibody, LAT antibody, lat antibody, Lat antibody

Background: LAT1, LAT, linker for activation of T cells, pp36, 36 kDa phospho-tyrosine adaptor protein, linker for activation of T-cells family member 1

Gene ID: 27040

NCBI Accession: NP_055202, NP_001014987, NP_001014989, NP_001014988

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway