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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||25 Antibodies||29 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies||17 Antibodies|
|Immunofluorescence (IF)||14 Antibodies||14 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||39 Antibodies||39 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Western Blotting (WB)||67 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Antigen||Linker For Activation of T Cells (LAT) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This LAT antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
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Product Details anti-LAT AntibodyTarget Details LAT Application Details Handling References for anti-LAT Antibody (ABIN238425) Images
|Specificity||The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.|
|Cross-Reactivity (Details)||Species reactivity (tested):Mouse and Rat.|
|Characteristics||Synonyms: pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-CellsFamily Member 1|
|Purification||Protein A Affinity Chromatography.|
|Immunogen||Recombinant rat LAT.|
Target Details LATProduct Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN238425) Images back to top
|Alternative Name||LAT (LAT Antibody Abstract)|
|Background||LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcεRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36|
|Research Area||Signaling, Adaptive Immunity|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN238425) Images back to top
Western Blotting: 5-10 μg/mLPositive control: mouse spleen. Immunoprecipitation. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN238425) Images back to top
|Buffer||PBS, pH 7.4, 0,05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-LAT Antibody (ABIN238425)Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007
Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007
Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, 2006
Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004
Tolar, T?mová, Dráber: "New monoclonal antibodies recognizing the adaptor protein LAT." in: Folia biologica, Vol. 47, Issue 6, pp. 215-7, 2001
ImagesProduct Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN238425) back to top