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anti-Human PKC theta antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended PKC theta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, theta (PRKCQ) Antibodies
  • A130035A12Rik
  • AW494342
  • nPKC-theta
  • PKC-0
  • PKC-theta
  • Pkcq
  • PKCtheta
  • PRKCT
Epitope
pThr538
44
41
39
9
9
8
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
208
121
111
4
4
4
4
Host
Mouse
210
13
3
1
Clonality
Monoclonal
Conjugate
This PKC theta antibody is un-conjugated
5
5
5
4
4
4
4
3
3
3
3
3
3
2
2
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
155
113
74
35
34
34
7
5
4
2
2
1
1
1
Supplier
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Catalog No. ABIN968924
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN967738 IHC (f) IF IP WB Mouse IgG2a, kappa AA 21-217 Log in to see 27-PKCtheta 5
0.0008709465 ABIN967739 IHC (f) IF IP WB Mouse IgG2a, kappa AA 21-217 Log in to see 27-PKCtheta 5

Top referenced anti-PKC theta antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-PKC theta Antibodies

Application / Reactivity Human
ELISA 72 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 35 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 111 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 149 Antibodies

General

Antigen Protein Kinase C, theta (PRKCQ) Antibodies
Epitope pThr538
(44), (41), (39), (9), (9), (8), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(208), (121), (111), (4), (4), (4), (4)
Host Mouse
(210), (13), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC theta antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(155), (113), (74), (35), (34), (34), (7), (5), (4), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-PKC theta Antibody

Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN968924) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human PKCtheta Peptide
Clone 19-PKC
Isotype IgG2a

Target Details PKC theta

Product Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN968924) Images back to top
Antigen
Alternative Name PKC theta (PRKCQ Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. Three categories exist, conventional PKC (cPKC), novel PKC (nPKC), and atypical PKC (aPKC). These proteins are products of multiple genes and alternative splicing and have different modes of activation. For example, cPKC's members (alpha, betaI, betaII, and gamma) are calcium activated, phospholipid-dependent serine/threonine specific enzymes which can also be activated by phorbol esters. However, the novel PKC (nPKC) subfamily members (delta , epsilon, pi, and theta isoforms) and the atypical PKC (PKC) subfamily members (zeta , í , and lambda isoforms) are Ca[2+] independent. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta RNA is readily detectable in skeletal muscle, lung, and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggests that it may have a role in growth and differentiation processes of these cells.
The 9/PKC monoclonal antibody recognizes the phosphorylated threonine 538 (pT538) of human PKCtheta.
Molecular Weight 79 kDa
Research Area Cancer
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN968924) Images back to top
Comment

Related Products: ABIN967738

Restrictions For Research Use only

Handling

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN968924) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-PKC theta Antibody (ABIN968924)

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Product cited in:

Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, 1990

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988

Images

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN968924) back to top
Supplier Images
 image for anti-PKC theta antibody (Protein Kinase C, theta) (pThr538) (ABIN968924) Jurkat cells were treated with Anti-CD3 and were then either left untreated (lane 1) ...