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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||35 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies|
|Immunohistochemistry (IHC)||111 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||33 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|Western Blotting (WB)||149 Antibodies|
|Antigen||Protein Kinase C, theta (PRKCQ) Antibodies|
|Conjugate||This PKC theta antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-PKC theta AntibodyTarget Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN968924) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Phosphorylated Human PKCtheta Peptide|
Target Details PKC thetaProduct Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN968924) Images back to top
|Alternative Name||PKC theta (PRKCQ Antibody Abstract)|
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. Three categories exist, conventional PKC (cPKC), novel PKC (nPKC), and atypical PKC (aPKC). These proteins are products of multiple genes and alternative splicing and have different modes of activation. For example, cPKC's members (alpha, betaI, betaII, and gamma) are calcium activated, phospholipid-dependent serine/threonine specific enzymes which can also be activated by phorbol esters. However, the novel PKC (nPKC) subfamily members (delta , epsilon, pi, and theta isoforms) and the atypical PKC (PKC) subfamily members (zeta , í , and lambda isoforms) are Ca[2+] independent. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta RNA is readily detectable in skeletal muscle, lung, and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggests that it may have a role in growth and differentiation processes of these cells.
The 9/PKC monoclonal antibody recognizes the phosphorylated threonine 538 (pT538) of human PKCtheta.
|Molecular Weight||79 kDa|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN968924) Images back to top
Related Products: ABIN967738
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN968924) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-PKC theta Antibody (ABIN968924)Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
|Product cited in:||
Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, 1990
Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988
ImagesProduct Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN968924) back to top