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anti-Human PKC theta antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PKC theta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, theta (PRKCQ) Antibodies
  • A130035A12Rik
  • AW494342
  • nPKC-theta
  • PKC-0
  • PKC-theta
  • Pkcq
  • PKCtheta
  • PRKCT
Epitope
C-Term
41
41
36
10
9
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
194
117
108
4
4
4
4
Host
Rabbit
202
7
3
1
Clonality
Polyclonal
Conjugate
This PKC theta antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
150
107
72
34
32
28
5
3
2
2
1
1
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Catalog No. ABIN359074
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32227957 ABIN498699 IF IHC (p) WB Rabbit pSer676 Log in to see Polyclonal
0.32227957 ABIN498658 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.00090133876 ABIN197566 IF IHC (p) WB Rabbit AA 674-678 Log in to see Polyclonal 3
0.00090133876 ABIN197070 IF IHC (p) WB Rabbit pSer676 Log in to see Polyclonal 3
0.00090133876 ABIN196902 IHC (p) Rabbit pSer695 Log in to see Polyclonal 2
0.00090133876 ABIN197400 IHC (p) WB Rabbit AA 693-697 Log in to see Polyclonal 2
0.00090133876 ABIN1501904 IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.00090133876 ABIN391017 IHC (p) WB Rabbit Ig AA 318-348, C-Term Log in to see Polyclonal
0.00090133876 ABIN2888514 IHC (p) ELISA WB Rabbit IgG pSer676 Log in to see Polyclonal
0.00090133876 ABIN2879256 IHC (p) ELISA WB Rabbit IgG AA 643-692 Log in to see Polyclonal
0.00090133876 ABIN317883 IHC (p) WB Rabbit Log in to see Polyclonal
0.00090133876 ABIN2888771 IHC (p) ELISA Rabbit IgG AA 657-706 Log in to see Polyclonal
0.00090133876 ABIN711746 IF (p) IHC (p) WB Rabbit IgG pThr538 Log in to see Polyclonal
0.00090133876 ABIN2879457 IF IHC (p) WB Rabbit Asn670, AA 640-690 Log in to see Polyclonal
0.00090133876 ABIN754740 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.00090133876 ABIN754747 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.00090133876 ABIN711718 IHC (p) WB Biotin Rabbit IgG pSer676 Log in to see Polyclonal
0.00090133876 ABIN711725 IHC (p) WB HRP Rabbit IgG pSer676 Log in to see Polyclonal
0.00090133876 ABIN1841104 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.00090133876 ABIN754738 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-PKC theta antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-PKC theta Antibodies

Application / Reactivity Human
ELISA 70 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 106 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 145 Antibodies

General

Antigen Protein Kinase C, theta (PRKCQ) Antibodies
Epitope C-Term
(41), (41), (36), (10), (9), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(194), (117), (108), (4), (4), (4), (4)
Host Rabbit
(202), (7), (3), (1)
Clonality
Polyclonal
Conjugate This PKC theta antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(150), (107), (72), (34), (32), (28), (5), (3), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-PKC theta Antibody

Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN359074) Images
Specificity This antibody reacts to PKC theta.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
Isotype Ig

Target Details PKC theta

Product Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN359074) Images back to top
Antigen
Alternative Name PRKCQ / PRKCT (PRKCQ Antibody Abstract)
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PKC theta is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.Synonyms: PKC theta, PKC-theta, Protein kinase C theta type, Protein kinase C theta type, nPKC-theta
Gene ID 5588, 9606
UniProt Q04759
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN359074) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN359074) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PKC theta Antibody (ABIN359074)

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Background publications

Carrasco, Merida: "Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes." in: Molecular biology of the cell, Vol. 15, Issue 6, pp. 2932-42, 2004

Sun, Mao, Rao et al.: "Association of CBFA2 mutation with decreased platelet PKC-theta and impaired receptor-mediated activation of GPIIb-IIIa and pleckstrin phosphorylation: proteins regulated by CBFA2 play a role in ..." in: Blood, Vol. 103, Issue 3, pp. 948-54, 2004

Burchfield, Lennard, Narasimhan, Hughes, Wasinger, Corthals, Okuda, Kondoh, Biden, Schmitz-Peiffer: "Akt mediates insulin-stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta." in: The Journal of biological chemistry, Vol. 279, Issue 18, pp. 18623-32, 2004

Ishaq, DeGray, Natarajan: "Protein kinase C theta modulates nuclear receptor-corepressor interaction during T cell activation." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39296-302, 2003

Cipriani, Knowles, Chen, Battistini, Brosnan: "Involvement of classical and novel protein kinase C isoforms in the response of human V gamma 9V delta 2 T cells to phosphate antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 10, pp. 5761-70, 2002

Images

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN359074) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PKC theta antibody (Protein Kinase C, theta) (C-Term) (ABIN359074) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-PKC theta antibody (Protein Kinase C, theta) (C-Term) (ABIN359074) Western blot analysis of anti-PKC theta Pab in placenta lysate. PKC theta (Arrow) was...