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anti-Rat (Rattus) PKC theta antibody for Western Blotting

Recommended PKC theta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, theta (PRKCQ) Antibodies
  • A130035A12Rik
  • AW494342
  • nPKC-theta
  • PKC-0
  • PKC-theta
  • Pkcq
  • PKCtheta
  • PRKCT
Epitope
pThr538
44
41
39
9
9
8
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
207
119
109
4
4
4
4
Host
Rabbit
208
14
3
1
Clonality
Polyclonal
Conjugate
This PKC theta antibody is un-conjugated
5
5
5
4
4
4
4
3
3
3
3
3
3
2
2
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
154
111
73
34
34
34
7
5
4
3
2
1
1
1
Supplier
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Catalog No. ABIN1531465
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN492623 WB Mouse IgG1 C-Term Log in to see 1C2
0.13398378 ABIN1531951 IHC ELISA WB Rabbit IgG pSer676 Log in to see Polyclonal
0.13398378 ABIN1532479 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN1532983 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN498699 IF IHC (p) WB Rabbit pSer676 Log in to see Polyclonal
0.13398378 ABIN2705132 IC IF IHC WB Rabbit C-Term, pSer676 Log in to see Polyclonal
0.13398378 ABIN498658 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.13398378 ABIN1870528 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal
0.13398378 ABIN1870527 IHC WB Rabbit IgG pSer695 Log in to see Polyclonal
0.13398378 ABIN2467873 WB Chicken AA 610-673 Log in to see Polyclonal
0.13398378 ABIN2499426 ELISA WB Rabbit IgG Ser676 Log in to see Polyclonal
0.13398378 ABIN2499427 IHC ELISA WB Rabbit IgG pSer676 Log in to see Polyclonal
0.13398378 ABIN3186479 ELISA IHC WB Rabbit IgG Thr319 Log in to see Polyclonal
0.13398378 ABIN2425053 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal
0.13398378 ABIN2424917 IHC WB Rabbit IgG pSer695 Log in to see Polyclonal
0.13398378 ABIN2425229 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN1844808 IHC WB Rabbit pSer676 Log in to see Polyclonal
0.13398378 ABIN1844806 WB Rabbit Log in to see Polyclonal
0.13398378 ABIN273681 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal
0.13398378 ABIN273680 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal

Similar anti-PKC theta Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 23 Antibodies 25 Antibodies 72 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 23 Antibodies 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 52 Antibodies 59 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 25 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 72 Antibodies 79 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Protein Kinase C, theta (PRKCQ) Antibodies
Epitope pThr538
(44), (41), (39), (9), (9), (8), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(207), (119), (109), (4), (4), (4), (4)
Host Rabbit
(208), (14), (3), (1)
Clonality
Polyclonal
Conjugate This PKC theta antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(154), (111), (73), (34), (34), (34), (7), (5), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PKC theta Antibody

Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN1531465) Images
Specificity PKC thet (Phospho-Thr538) Antibody detects endogenous levels of PKC thet only when phosphorylated at Thr538.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538.
Isotype IgG

Target Details PKC theta

Product Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN1531465) Images back to top
Antigen
Alternative Name PKC theta (PRKCQ Antibody Abstract)
Background Molecular weight of target protein: 81 kDa
Synonyms: KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT, Protein kinase C, theta type, kinase PKC-theta, nPKC-theta
Molecular Weight 81 kDa
Gene ID 5588
OMIM 600448
UniProt Q04759
Research Area Cancer
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN1531465) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.498570 (NCBI Gene Symbol: PRKCQ)

Restrictions For Research Use only

Handling

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN1531465) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-PKC theta Antibody (ABIN1531465)

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Background publications

Deloukas, Earthrowl, Grafham, Rubenfield, French, Steward, Sims, Jones, Searle, Scott, Howe, Hunt, Andrews, Gilbert, Swarbreck, Ashurst, Taylor, Battles, Bird, Ainscough, Almeida, Ashwell, Ambrose et al.: "The DNA sequence and comparative analysis of human chromosome 10. ..." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004

Images

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN1531465) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC theta antibody (Protein Kinase C, theta) (pThr538) (ABIN1531465) Western blot analysis of extracts from Jurkat cells, using PKC thet (Phospho-Thr538) ...
Immunohistochemistry (IHC) image for anti-PKC theta antibody (Protein Kinase C, theta) (pThr538) (ABIN1531465) Immunohistochemistry analysis of paraffin-embedded human brain, using PKC thet (Phosp...