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anti-Human VAV1 antibody for Flow Cytometry

Recommended VAV1 Antibody (supplied by: Log in to see )

Antigen
Vav 1 Oncogene (VAV1) Antibodies
  • VAV
  • Vav
  • vav-T
Epitope
AA 121-324
34
21
19
15
13
11
5
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
137
63
61
1
1
1
Host
Mouse
125
12
Clonality
Monoclonal
Conjugate
This VAV1 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
115
55
23
12
9
8
5
3
1
1
1
Supplier
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Catalog No. ABIN969581
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN1845807 FACS ELISA WB Mouse IgG1 AA 121-324 Log in to see
0.0010666476 ABIN1490879 FACS ELISA WB Mouse IgG1 Log in to see 2E11
0.0010666476 ABIN229274 FACS IP ELISA WB Rabbit IgG Log in to see Polyclonal
0.0010666476 ABIN2384810 FACS ELISA WB Mouse IgG1 Log in to see 13B793
0.0010666476 ABIN2384811 FACS IP ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-VAV1 antibody for Flow Cytometry

Similar anti-VAV1 Antibodies

Application / Reactivity Human
ELISA 56 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 25 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 119 Antibodies

General

Antigen Vav 1 Oncogene (VAV1) Antibodies
Epitope AA 121-324
(34), (21), (19), (15), (13), (11), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(137), (63), (61), (1), (1), (1)
Host Mouse
(125), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VAV1 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(115), (55), (23), (12), (9), (8), (5), (3), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-VAV1 Antibody

Target Details VAV1 Application Details Handling References for anti-VAV1 Antibody (ABIN969581) Images
Purification Purified
Immunogen Purified recombinant fragment of human VAV1 (AA 121-324) expressed in E. coli.
Clone 2-00E-11
Isotype IgG1

Target Details VAV1

Product Details anti-VAV1 Antibody Application Details Handling References for anti-VAV1 Antibody (ABIN969581) Images back to top
Antigen
Alternative Name VAV1 (VAV1 Antibody Abstract)
Background The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.
Molecular Weight 98.3 kDa
Gene ID 7409
HGNC 7409
Research Area Cancer, Transcription Factors
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-VAV1 Antibody Target Details VAV1 Handling References for anti-VAV1 Antibody (ABIN969581) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/250, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-VAV1 Antibody Target Details VAV1 Application Details References for anti-VAV1 Antibody (ABIN969581) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-VAV1 Antibody (ABIN969581)

Product Details anti-VAV1 Antibody Target Details VAV1 Application Details Handling Images back to top
Product cited in:

Bertagnolo, Nika, Brugnoli, Bonora, Grassilli, Pinton, Capitani: "Vav1 is a crucial molecule in monocytic/macrophagic differentiation of myeloid leukemia-derived cells." in: Cell and tissue research, Vol. 345, Issue 1, pp. 163-75, 2011

Yin, Wan, Li, Du, Zhang, Zhou, Cao: "The distinct role of guanine nucleotide exchange factor Vav1 in Bcl-2 transcription and apoptosis inhibition in Jurkat leukemia T cells." in: Acta pharmacologica Sinica, Vol. 32, Issue 1, pp. 99-107, 2011

Images

Product Details anti-VAV1 Antibody Target Details VAV1 Application Details Handling References for anti-VAV1 Antibody (ABIN969581) back to top
Supplier Images
ELISA image for anti-VAV1 antibody (Vav 1 Oncogene) (AA 121-324) (ABIN969581) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-VAV1 antibody (Vav 1 Oncogene) (AA 121-324) (ABIN969581) Figure 2: Flow cytometric analysis of HeLa cells using VAV1 mouse mAb (green) and neg...
Western Blotting (WB) image for anti-VAV1 antibody (Vav 1 Oncogene) (AA 121-324) (ABIN969581) Figure 3: Western blot analysis using VAV1 mouse mAb against Jurkat (1) cell lysate.
Western Blotting (WB) image for anti-VAV1 antibody (Vav 1 Oncogene) (AA 121-324) (ABIN969581) Figure 4: Western blot analysis using VAV1 mAb against human VAV1 recombinant protein...
Western Blotting (WB) image for anti-VAV1 antibody (Vav 1 Oncogene) (AA 121-324) (ABIN969581) Figure 5: Western blot analysis using VAV1 mAb against HEK293 (1) and VAV1 (AA: 121-3...