anti-Human VAV3 antibody for Western Blotting

Recommended VAV3 Antibody (supplied by: Log in to see )

Antigen
Vav 3 Oncogene (VAV3) Antibodies
  • RGD1565941
  • A530094I06Rik
  • AA986410
  • Idd18.1
  • vav 3 guanine nucleotide exchange factor
  • vav 3 oncogene
  • VAV3
  • Vav3
Epitope
both Isoforms
15
15
12
10
5
5
2
2
2
2
1
1
1
1
1
Reactivity
Human
86
50
31
2
2
Host
Goat
71
18
Clonality
Polyclonal
Conjugate
This VAV3 antibody is un-conjugated
5
5
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Western Blotting (WB)
62
33
26
10
10
7
3
2
2
1
Supplier
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Catalog No. ABIN268548
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1403036 ABIN614934 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal
3.1403036 ABIN3182717 ELISA IHC WB Rabbit IgG pTyr173 Log in to see Polyclonal
3.1403036 ABIN3180941 ELISA WB Rabbit IgG Tyr542 Log in to see Polyclonal
3.1403036 ABIN2466429 ELISA WB Goat Internal Region Log in to see Polyclonal
3.1403036 ABIN1860913 ICC IHC IP WB Rabbit IgG AA 183-380 Log in to see Polyclonal
3.1403036 ABIN1860914 ICC IHC IP WB Rabbit IgG AA 398-583 Log in to see Polyclonal
3.1403036 ABIN2560712 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.1403036 ABIN307104 WB Rabbit IgG pTyr173 Log in to see 5
3.1403036 ABIN1847856 ELISA WB Rabbit Internal Region, pTyr173 Log in to see Polyclonal
1 ABIN185278 ELISA WB Goat Internal Region Log in to see Polyclonal 1
1 ABIN1450791 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 2
1 ABIN461931 IHC (p) ELISA WB Goat AA 567-578 Log in to see Polyclonal
1 ABIN2568523 WB Rabbit IgG pTyr173 Log in to see Polyclonal
1 ABIN706421 IF (p) IHC (p) WB Rabbit IgG AA 710-760 Log in to see Polyclonal
1 ABIN210658 ICC IP IHC WB Rabbit IgG AA 566-591 Log in to see Polyclonal
1 ABIN1740680 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN2755764 WB Rabbit Center Log in to see Polyclonal
1 ABIN706430 IHC (p) WB HRP Rabbit IgG AA 710-760 Log in to see Polyclonal
1 ABIN1457880 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN2570235 ELISA WB Biotin Rabbit IgG AA 398-583 Log in to see Polyclonal

Top referenced anti-VAV3 antibody for Western Blotting

Similar anti-VAV3 Antibodies

Application / Reactivity Human
ELISA 34 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 60 Antibodies

General

Antigen Vav 3 Oncogene (VAV3) Antibodies
Epitope both Isoforms
(15), (15), (12), (10), (5), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(86), (50), (31), (2), (2)
Host Goat
(71), (18)
Clonality
Polyclonal
Conjugate This VAV3 antibody is un-conjugated
(5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(62), (33), (26), (10), (10), (7), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-VAV3 Antibody

Target Details VAV3 Application Details Handling ProductDetails: References for anti-VAV3 antibody (ABIN268548) Images
Specificity This antibody is expected to recognize both reported isoforms (NP_006104.4, NP_001073343.1).
Purification Immunogen affinity purified
Immunogen Peptide with sequence CSGEQGTLKLPEK corresponding to internal region according to NP_006104.4, NP_001073343.1.

Target Details VAV3

Product Details anti-VAV3 Antibody Application Details Handling ProductDetails: References for anti-VAV3 antibody (ABIN268548) Images back to top
Antigen
Alternative Name VAV3 (VAV3 Antibody Abstract)
Background Gene Symbol: VAV3
Gene ID 10451
Research Area Cancer, Cell Cycle
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-VAV3 Antibody Target Details VAV3 Handling ProductDetails: References for anti-VAV3 antibody (ABIN268548) Images back to top
Application Notes Western Blot 0.3 - 1 μg/mL, Peptide ELISA Detection limit 1:64000WB: Approx. 90 kDa band observed in human bone marrow lysates (calculated MW of 97.8 kDa band according to NP_006104).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for VAV3 Antibody Lysis:Cell pellets were washed with ice-cold PBS. 1 mL of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 mL microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was then added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1 % Triton X-100, 1 % Sodium deoxycholate, 0.1 % SDS). Tissue Lysis:Tissue chunks were weighed and cut into approx 1mm cubes using a razor blade. The tissue was transferred to a handheld homogenizer and 3 mL of ice-cold RIPA buffer was added per 1g of tissue. The tissue was gently homogenised over 20 minutes on ice. The resulting lysate was aliquotted into 1.5 mL microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1 % Triton X-100, 1 % Sodium deoxycholate, 0.1 % SDS). SDS PAGE:Samples were run at 200V constant on a 12 % acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5 % (v/v) 2-ME added and were boiled for 3 minutes. Transfer:We used a Biorad Mini Trans-Blot, constant 100 V for 1 hour. Transfer
Restrictions For Research Use only

Handling

Product Details anti-VAV3 Antibody Target Details VAV3 Application Details ProductDetails: References for anti-VAV3 antibody (ABIN268548) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

ProductDetails: References for anti-VAV3 antibody (ABIN268548)

Product Details anti-VAV3 Antibody Target Details VAV3 Application Details Handling Images back to top
Product cited in:

Movilla, Bustelo: "Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins." in: Molecular and cellular biology, Vol. 19, Issue 11, pp. 7870-85, 1999

Images

Product Details anti-VAV3 Antibody Target Details VAV3 Application Details Handling ProductDetails: References for anti-VAV3 antibody (ABIN268548) back to top
Supplier Images
Western Blotting (WB) image for anti-VAV3 antibody (Vav 3 Oncogene) (both Isoforms) (ABIN268548) Western Blot: VAV3 Antibody [ABIN268548] - Analysis of VAV3 in human bone marrow lysa...