anti-Cow (Bovine) ZAP70 antibody for Western Blotting

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
N-Term
79
53
49
37
28
24
23
15
12
12
10
10
10
8
8
8
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
580
218
142
13
11
10
9
8
6
5
4
3
1
Host
Rabbit
314
243
16
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
31
24
20
19
13
9
9
7
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
1
Application
Western Blotting (WB)
318
172
169
162
158
83
52
49
23
7
5
5
4
4
4
3
3
2
2
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Catalog No. ABIN2788712
$ 289.00
Plus shipping costs $45.00
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General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope N-Term
(79), (53), (49), (37), (28), (24), (23), (15), (12), (12), (10), (10), (10), (8), (8), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
(580), (218), (142), (13), (11), (10), (9), (8), (6), (5), (4), (3), (1)
Host Rabbit
(314), (243), (16), (8), (3)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(31), (24), (20), (19), (13), (9), (9), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1)
Application Western Blotting (WB)
(318), (172), (169), (162), (158), (83), (52), (49), (23), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN986321
Sequence MRKKQIDVAI KVLKQGTEKA DTEEMMREAQ IMHQLDNPYI VRLIGVCQAE
Predicted Reactivity Cow: 79%, Guinea Pig: 79%, Human: 100%, Mouse: 86%, Rat: 79%
Characteristics This is a rabbit polyclonal antibody against ZAP70. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human ZAP70

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Alias Symbols: FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70
Protein Interaction Partner: UBC, CSK, PLCG1, ZAP70, CD247, CD3E, LAT, NDUFB8, ERBB3, EGFR, ANAPC11, ASB3, PTPN22, NR0B2, SHC1, GRB2, APP, LCK, CBL, CBLC, LCP2, CBLB, PIK3R1, CD38, HSPA4, TUBB, TUBA1A, VAV1, TUBA1B, tat, NFAM1, DEF6, ACP1, LAX1, FCRL3, PTPN3, DBNL, CARD11, SLA2, SLAM
Protein Size: 312
Molecular Weight 34 kDa
Gene ID 7535
NCBI Accession NM_207519, NP_997402
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 312 AA

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (N-Term) antibody (ABIN2788712) WB Suggested Anti-ZAP70 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Lung