anti-Human ZAP70 antibody for Flow Cytometry

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Reactivity
Human, Mouse (Murine)
558
218
143
14
11
10
9
8
6
6
4
3
1
Host
Mouse
310
225
16
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is conjugated to PE
30
22
19
18
13
8
8
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
315
170
153
145
144
70
52
48
23
7
5
5
4
4
4
3
3
2
2
Supplier
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Available images

Catalog No. ABIN2663949
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.340045 ABIN2662039 FACS FITC Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
13.340045 ABIN438732 FACS IP Rabbit pTyr319 Log in to see Polyclonal
1 ABIN658066 FACS IHC (p) WB Rabbit Ig AA 276-304, Center Log in to see Polyclonal 2
1 ABIN238530 FACS IF WB Mouse IgG1 C-Term Log in to see ZAP-03 4
1 ABIN125756 ICC FACS WB Mouse IgG1 C-Term Log in to see ZAP-03 3
1 ABIN205364 FACS IP WB Mouse IgG1 AA 282-307 Log in to see 1E7-2
1 ABIN180999 FACS PE Mouse IgG1 AA 280-309 Log in to see SBZAP
1 ABIN962077 FACS IHC (p) WB Mouse IgG1 C-Term Log in to see ZAP-03
1 ABIN2665463 FACS Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 5
1 ABIN2477168 FACS WB Mouse IgG1 C-Term Log in to see ZAP-03 2
1 ABIN2477170 FACS FITC Mouse IgG1 AA 280-309 Log in to see SBZAP 2
1 ABIN2477172 FACS PE Mouse IgG1 AA 280-309 Log in to see SBZAP 2
1 ABIN207127 Inhibition FACS ICC WB Mouse IgG1 C-Term Log in to see ZAP-03
1 ABIN1723985 FACS IHC (p) WB Mouse IgG1 C-Term Log in to see ZAP-03
1 ABIN598473 FACS FITC Mouse IgG1 AA 280-309 Log in to see
1 ABIN598474 FACS FITC Mouse IgG1 AA 280-309 Log in to see
1 ABIN598476 FACS PE Mouse IgG1 AA 280-309 Log in to see
1 ABIN4366582 FACS IF IHC (p) Mouse IgG AA 1-254 Log in to see ZAP70-528-2F3-2
1 ABIN4366573 FACS IF IHC (p) SimWes Mouse IgG2a kappa AA 1-254 Log in to see 2F3-2
1 ABIN4366581 FACS IF IHC (p) Mouse IgG AA 1-254 Log in to see ZAP70-528-2F3-2

Top referenced anti-ZAP70 antibody for Flow Cytometry

  • Human Monoclonal ZAP70 Primary Antibody for ICC, FACS - ABIN125756 (3 Publications): Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. in The Journal of biological chemistry 2011 (PubMed)

Similar anti-ZAP70 Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies 5 Antibodies
ELISA 48 Antibodies 167 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 21 Antibodies 154 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 67 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Western Blotting (WB) 131 Antibodies
Blocking Antibody (Inhibition) 2 Antibodies
FLISA 2 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Reactivity Human, Mouse (Murine)
(558), (218), (143), (14), (11), (10), (9), (8), (6), (6), (4), (3), (1)
Host Mouse
(310), (225), (16), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is conjugated to PE
(30), (22), (19), (18), (13), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(315), (170), (153), (145), (144), (70), (52), (48), (23), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
Pubmed 4 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling ProductDetails: References for anti-ZAP70 antibody (ABIN2663949) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Clone 1E7-2
Isotype IgG1 kappa, IgG1

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling ProductDetails: References for anti-ZAP70 antibody (ABIN2663949) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling ProductDetails: References for anti-ZAP70 antibody (ABIN2663949) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details ProductDetails: References for anti-ZAP70 antibody (ABIN2663949) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

ProductDetails: References for anti-ZAP70 antibody (ABIN2663949)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Crespo, Bosch, Villamor, Bellosillo, Colomer, Rozman, Marcé, López-Guillermo, Campo, Montserrat: "ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia." in: The New England journal of medicine, Vol. 348, Issue 18, pp. 1764-75, 2003

Qian, Lev, van Oers, Dikic, Schlessinger, Weiss: "Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling." in: The Journal of experimental medicine, Vol. 185, Issue 7, pp. 1253-9, 1997

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994

Chan, Iwashima, Turck, Weiss: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling ProductDetails: References for anti-ZAP70 antibody (ABIN2663949) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa)  (PE) (ABIN2663949) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (PE)