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anti-Human ZAP70 antibody for Flow Cytometry

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Reactivity
Human, Mouse (Murine)
530
185
121
4
4
3
2
2
2
Host
Mouse
298
212
15
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is conjugated to FITC
30
24
18
16
10
8
8
7
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Flow Cytometry (FACS)
297
163
155
147
121
68
54
44
24
7
5
5
4
4
3
2
Supplier
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Catalog No. ABIN2662039
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2663949 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
0.30226976 ABIN438732 FACS IP Rabbit IgG pTyr319 Log in to see Polyclonal
0.30226976 ABIN2494335 FACS IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.0010482287 ABIN658066 FACS IHC (p) WB Rabbit Ig AA 276-304, Center Log in to see Polyclonal 2
0.0010482287 ABIN238530 FACS IF WB Mouse IgG1 C-Term Log in to see ZAP-03 4
0.0010482287 ABIN125756 ICC FACS WB Mouse IgG1 C-Term Log in to see ZAP-03 3
0.0010482287 ABIN962077 FACS IHC (p) WB Mouse IgG1 C-Term Log in to see ZAP-03
0.0010482287 ABIN153574 FACS IP ELISA WB Mouse IgG2a Cytoplasmic Domain Log in to see G3
0.0010482287 ABIN180999 FACS PE Mouse IgG1 AA 280-309 Log in to see SBZAP
0.0010482287 ABIN205364 FACS IP WB Mouse IgG1 AA 282-307 Log in to see 1E7-2
0.0010482287 ABIN2665463 FACS Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 5
0.0010482287 ABIN207127 FACS WB Mouse IgG1 C-Term Log in to see ZAP-03
0.0010482287 ABIN598473 FACS FITC Mouse IgG1 AA 280-309 Log in to see
0.0010482287 ABIN598474 FACS FITC Mouse IgG1 AA 280-309 Log in to see
0.0010482287 ABIN598476 FACS PE Mouse IgG1 AA 280-309 Log in to see
0.0010482287 ABIN1723985 FACS IHC (p) WB Mouse IgG1 C-Term Log in to see ZAP-03
0.0010482287 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Log in to see Polyclonal
0.0010482287 ABIN218908 FACS IP ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
0.0010482287 ABIN212049 FACS ELISA PE Mouse IgG1, kappa AA 280-309 Log in to see
0.0010482287 ABIN212048 FACS ELISA Biotin Mouse IgG1, kappa AA 280-309 Log in to see

Top referenced anti-ZAP70 antibody for Flow Cytometry

Similar anti-ZAP70 Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 295 Antibodies 109 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Immunoprecipitation (IP) 57 Antibodies 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 156 Antibodies 67 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunofluorescence (IF) 69 Antibodies 33 Antibodies
Immunocytochemistry (ICC) 25 Antibodies 19 Antibodies
Immunochromatography (IC) 7 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Reactivity Human, Mouse (Murine)
(530), (185), (121), (4), (4), (3), (2), (2), (2)
Host Mouse
(298), (212), (15), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is conjugated to FITC
(30), (24), (18), (16), (10), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Flow Cytometry (FACS)
(297), (163), (155), (147), (121), (68), (54), (44), (24), (7), (5), (5), (4), (4), (3), (2)
Pubmed 4 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) Images
Purification The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Clone 1E7-2
Isotype IgG1 kappa, IgG1

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-ZAP70 Antibody (ABIN2662039)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Crespo, Bosch, Villamor, Bellosillo, Colomer, Rozman, Marcé, López-Guillermo, Campo, Montserrat: "ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia." in: The New England journal of medicine, Vol. 348, Issue 18, pp. 1764-75, 2003

Qian, Lev, van Oers, Dikic, Schlessinger, Weiss: "Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling." in: The Journal of experimental medicine, Vol. 185, Issue 7, pp. 1253-9, 1997

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994

Chan, Iwashima, Turck, Weiss: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa)  (FITC) (ABIN2662039) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (FITC)