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anti-Human ZAP70 antibody for Immunocytochemistry

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Epitope
C-Term
50
47
45
25
22
21
21
20
15
15
15
14
12
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
456
171
119
4
4
3
2
2
2
Host
Mouse
296
140
15
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is un-conjugated
29
21
16
14
10
7
5
5
4
4
4
4
4
4
4
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
278
162
151
81
62
44
39
38
9
7
5
4
4
3
2
Supplier
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Catalog No. ABIN125756
$ 254.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001015949 ABIN210659 ICC IP WB Mouse IgG1 AA 282-307 Log in to see
0.001015949 ABIN1875382 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.001015949 ABIN447018 ICC IF IHC (p) IHC WB Rabbit Ser313 Log in to see Polyclonal
0.001015949 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Log in to see Polyclonal
0.001015949 ABIN198253 ICC FITC Mouse IgG2a AA 1-254 Log in to see
0.001015949 ABIN2405264 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.001015949 ABIN3200826 FACS ICC WB Mouse IgG1 C-Term Log in to see ZAP-03
0.001015949 ABIN2988348 ICC IF IHC WB Rabbit IgG pTyr319 Log in to see Polyclonal
0.001015949 ABIN2973695 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.001015949 ABIN3040296 ICC FACS IF WB Mouse IgG1 C-Term Log in to see

Top referenced anti-ZAP70 antibody for Immunocytochemistry

  • Human Monoclonal ZAP70 Primary Antibody for ICC, FACS - ABIN125756 (3 Publications): Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. in The Journal of biological chemistry 2011 (PubMed)

Similar anti-ZAP70 Antibodies

Application / Reactivity Human
ELISA 160 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 84 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 153 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies
Immunoprecipitation (IP) 42 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 278 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope C-Term
(50), (47), (45), (25), (22), (21), (21), (20), (15), (15), (15), (14), (12), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(456), (171), (119), (4), (4), (3), (2), (2), (2)
Host Mouse
(296), (140), (15), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(29), (21), (16), (14), (10), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
(278), (162), (151), (81), (62), (44), (39), (38), (9), (7), (5), (4), (4), (3), (2)
Pubmed 3 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN125756) Images
Specificity The antibody ZAP-03 reacts with ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag
Clone ZAP-03
Isotype IgG1

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN125756) Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN125756) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 2-5 µg/mL
Positive control: HPB-ALL human peripheral blood T cell leukemia cell line
Application note: intracellular Staining
Western Blotting: Recommended dilution: 0,5 µg/mL
Positive control: HPB-ALL human peripheral blood T cell leukemia cell line
Negative control: RAMOS human Burkitt lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1: 1) with reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
Application note: Reducing conditions. SDS-PAGE (11 % separating gel).

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN125756) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-ZAP70 Antibody (ABIN125756)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Product cited in:

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011

Background publications

Schneider, Smith, Liu, Bismuth, Rudd: "CTLA-4 disrupts ZAP70 microcluster formation with reduced T cell/APC dwell times and calcium mobilization." in: European journal of immunology, Vol. 38, Issue 1, pp. 40-7, 2007

Ishaq, DeGray, Natarajan: "Evidence for the involvement of tyrosine kinase ZAP 70 in nuclear retinoid receptor-dependent transactivation in T lymphocytes." in: The Journal of biological chemistry, Vol. 280, Issue 40, pp. 34152-8, 2005

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN125756) back to top
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (C-Term) (ABIN125756) Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell le...
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (C-Term) (ABIN125756) Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell le...