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anti-Human ZAP70 antibody for Immunohistochemistry

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Epitope
pTyr493
64
50
47
46
25
22
21
21
20
15
15
15
14
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
529
185
120
4
4
3
2
2
2
Host
Rabbit
297
212
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
31
24
18
16
10
8
8
7
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
295
161
153
148
120
68
54
44
24
7
5
5
4
4
3
2
Supplier
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Catalog No. ABIN1532002
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22919032 ABIN969464 IHC ELISA WB Mouse IgG1 Log in to see 3D2A4 3
0.22919032 ABIN1531416 IHC ELISA WB Rabbit IgG pTyr315 Log in to see Polyclonal
0.22919032 ABIN1532433 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22919032 ABIN1533039 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22919032 ABIN1533040 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22919032 ABIN1870702 IHC WB Rabbit IgG pTyr493 Log in to see Polyclonal
0.22919032 ABIN2501280 IHC Rabbit IgG Tyr493 Log in to see Polyclonal
0.22919032 ABIN2501284 IHC WB Rabbit IgG Tyr319 Log in to see Polyclonal
0.22919032 ABIN2501290 IHC ELISA WB Rabbit IgG Tyr319 Log in to see Polyclonal
0.22919032 ABIN2501281 IHC Rabbit IgG pTyr493 Log in to see Polyclonal
0.22919032 ABIN2464121 IHC ELISA WB Mouse IgG1 truncated Log in to see 3D2A4
0.22919032 ABIN2501291 IHC ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
0.22919032 ABIN2501286 IHC ELISA Rabbit IgG Internal Region Log in to see Polyclonal
0.22919032 ABIN2501285 IHC ELISA Rabbit IgG Tyr493 Log in to see Polyclonal
0.22919032 ABIN447018 ICC IF IHC (p) IHC WB Rabbit Ser313 Log in to see Polyclonal
0.22919032 ABIN258742 IHC (fro) IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 3D2A4
0.22919032 ABIN2469923 IHC WB Mouse full length Log in to see 11H40
0.22919032 ABIN2494335 FACS IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.22919032 ABIN2494336 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.22919032 ABIN2425381 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-ZAP70 antibody for Immunohistochemistry

Similar anti-ZAP70 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 23 Antibodies 40 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 19 Antibodies
Immunofluorescence (IF) 16 Antibodies 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 56 Antibodies 67 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 22 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 70 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr493
(64), (50), (47), (46), (25), (22), (21), (21), (20), (15), (15), (15), (14), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(529), (185), (120), (4), (4), (3), (2), (2), (2)
Host Rabbit
(297), (212), (15), (8), (3)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(31), (24), (18), (16), (10), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(295), (161), (153), (148), (120), (68), (54), (44), (24), (7), (5), (5), (4), (4), (3), (2)
Pubmed 2 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) Images
Specificity ZAP-70 (Phospho-Tyr493) Antibody detects endogenous levels of ZAP- 70 only when phosphorylated at Tyr493.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr493.
Isotype IgG

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background Molecular weight of target protein: 69 kDa
Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70
Molecular Weight 69 kDa
Gene ID 7535
OMIM 176947
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:4000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.234569 (NCBI Gene Symbol: ZAP70)

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-ZAP70 Antibody (ABIN1532002)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005

Chan, Iwashima, Turck, Weiss: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) back to top
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN1532002) Western blot analysis of extracts from Jurkat cells, using ZAP-70 (Phospho-Tyr493) An...
Immunohistochemistry (IHC) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN1532002) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-...