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anti-Mouse (Murine) ZAP70 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
pTyr493
62
49
48
37
28
23
22
14
13
12
10
10
10
8
8
8
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
530
215
140
14
11
10
9
8
6
6
4
3
1
Host
Rabbit
300
208
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
28
22
18
17
12
8
8
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
298
161
143
142
130
63
52
46
20
7
5
5
4
4
4
3
3
2
2
Supplier
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Catalog No. ABIN498719
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.763539 ABIN498718 IF IHC (p) WB Rabbit pTyr319 Log in to see Polyclonal
12.763539 ABIN498597 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN196886 IHC (p) WB Rabbit pTyr493 Log in to see Polyclonal 3
1 ABIN197386 IHC (p) Rabbit Tyr493 Log in to see Polyclonal 3
1 ABIN197384 IHC (p) WB Rabbit Tyr319 Log in to see Polyclonal 6
1 ABIN2888559 IHC (p) ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN2878972 IHC (p) ELISA Rabbit IgG AA 460-509 Log in to see Polyclonal
1 ABIN2854924 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN1740899 IHC (p) ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1823053 IHC (p) Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN746942 IHC (p) WB HRP Rabbit IgG pTyr315, pTyr319 Log in to see Polyclonal
1 ABIN746957 IHC (p) WB HRP Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN2893307 IF IHC (p) WB Rabbit pTyr319 Log in to see Polyclonal
1 ABIN2892964 IF IHC (p) WB Rabbit Ser313 Log in to see Polyclonal
1 ABIN2884499 ELISA IHC (p) Rabbit IgG AA 281-330 Log in to see Polyclonal
1 ABIN2884501 ELISA IHC (p) WB Rabbit IgG pTyr315 Log in to see Polyclonal
1 ABIN2893308 IHC (p) WB Rabbit pTyr493 Log in to see Polyclonal
1 ABIN1712430 IHC (p) WB HRP Rabbit IgG pTyr292 Log in to see Polyclonal
1 ABIN675677 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN746935 IHC (p) WB Biotin Rabbit IgG pTyr315, pTyr319 Log in to see Polyclonal

Top referenced anti-ZAP70 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-ZAP70 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 21 Antibodies 46 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 22 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 16 Antibodies
Immunofluorescence (IF) 16 Antibodies 32 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 80 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr493
(62), (49), (48), (37), (28), (23), (22), (14), (13), (12), (10), (10), (10), (8), (8), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(530), (215), (140), (14), (11), (10), (9), (8), (6), (6), (4), (3), (1)
Host Rabbit
(300), (208), (15), (8), (3)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(28), (22), (18), (17), (12), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(298), (161), (143), (142), (130), (63), (52), (46), (20), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Specificity This antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Tyr493.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
Molecular Weight approx. 70 kDa
Gene ID 7535
NCBI Accession NP_001070
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN498719) Immunohistochemistry (IHC) analyzes of p-ZAP-70 antibody in paraffin-embedded ...
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN498719) Western blot (WB) analyzes of p-ZAP-70 in extracts from Jurkat cells.