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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||21 Antibodies||46 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||22 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||16 Antibodies|
|Immunofluorescence (IF)||16 Antibodies||32 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||39 Antibodies||52 Antibodies|
|Immunohistochemistry (IHC)||54 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Western Blotting (WB)||78 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Antigen||zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ZAP70 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-ZAP70 AntibodyTarget Details ZAP70 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Tyr319.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details ZAP70Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
|Alternative Name||ZAP70 (ZAP70 Antibody Abstract)|
|Background||The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70|
|Molecular Weight||approx. 70 kDa|
|Research Area||Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling back to top