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anti-Mouse (Murine) ZAP70 antibody for Western Blotting

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
pTyr493
65
52
50
45
27
23
23
22
20
15
15
15
14
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
546
188
124
4
4
3
2
2
2
Host
Rabbit
310
216
16
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
30
23
18
17
11
8
8
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
299
166
153
146
122
67
52
44
21
7
5
5
4
4
4
3
2
Options
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Catalog No. ABIN1532002
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.022232 ABIN967848 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
3.022232 ABIN967849 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
3.022232 ABIN1109533 EIA WB Goat Internal Region Log in to see Polyclonal
3.022232 ABIN1531415 ELISA WB Rabbit IgG pTyr292 Log in to see Polyclonal
3.022232 ABIN1531416 IHC ELISA WB Rabbit IgG pTyr315 Log in to see Polyclonal
3.022232 ABIN1532001 ELISA WB Rabbit IgG pTyr319 Log in to see Polyclonal
3.022232 ABIN967585 IHC (f) WB Mouse IgG1, kappa pTyr493 Log in to see 1a-Zap70 3
3.022232 ABIN3187521 ELISA WB Rabbit IgG Tyr349 Log in to see Polyclonal
3.022232 ABIN498718 IF IHC (p) WB Rabbit pTyr319 Log in to see Polyclonal
3.022232 ABIN1532432 ELISA WB Rabbit IgG Log in to see Polyclonal
3.022232 ABIN1533039 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.022232 ABIN498719 IF IHC (p) WB Rabbit pTyr493 Log in to see Polyclonal
3.022232 ABIN1870700 IF IHC WB Rabbit IgG pTyr319 Log in to see Polyclonal
3.022232 ABIN1870702 IHC WB Rabbit IgG pTyr493 Log in to see Polyclonal
3.022232 ABIN2957797 IC IF IHC WB Rabbit pTyr319 Log in to see Polyclonal
3.022232 ABIN2957799 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.022232 ABIN2788711 WB Rabbit Middle Region Log in to see Polyclonal
3.022232 ABIN2707263 WB Rabbit pTyr315 Log in to see Polyclonal
3.022232 ABIN498597 IF IHC (p) WB Rabbit Log in to see Polyclonal
3.022232 ABIN2854924 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal

Top referenced anti-ZAP70 antibody for Western Blotting

Similar anti-ZAP70 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 21 Antibodies 37 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 15 Antibodies
Immunofluorescence (IF) 15 Antibodies 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 53 Antibodies 63 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 67 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Flow Cytometry (FACS) 18 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr493
(65), (52), (50), (45), (27), (23), (23), (22), (20), (15), (15), (15), (14), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(546), (188), (124), (4), (4), (3), (2), (2), (2)
Host Rabbit
(310), (216), (16), (8), (3)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(30), (23), (18), (17), (11), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(299), (166), (153), (146), (122), (67), (52), (44), (21), (7), (5), (5), (4), (4), (4), (3), (2)
Pubmed 2 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) Images
Specificity ZAP-70 (Phospho-Tyr493) Antibody detects endogenous levels of ZAP- 70 only when phosphorylated at Tyr493.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr493.
Isotype IgG

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background Molecular weight of target protein: 69 kDa
Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70
Molecular Weight 69 kDa
Gene ID 7535
OMIM 176947
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:4000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.234569 (NCBI Gene Symbol: ZAP70)

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-ZAP70 Antibody (ABIN1532002)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005

Chan, Iwashima, Turck, Weiss: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) back to top
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN1532002) Western blot analysis of extracts from Jurkat cells, using ZAP-70 (Phospho-Tyr493) An...
Immunohistochemistry (IHC) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN1532002) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-...