anti-Human Adenomatous Polyposis Coli antibody for ELISA

Recommended Adenomatous Polyposis Coli Antibody (supplied by: Log in to see )

Antigen
Adenomatous Polyposis Coli (APC) Antibodies
  • dp2
  • dp3
  • fap
  • fpc
  • xapc
  • dp2.5
  • AI047805
  • AU020952
  • AW124434
  • CC1
  • Min
  • mAPC
  • BTPS2
  • DP2
  • DP2.5
  • DP3
  • GS
  • PPP1R46
  • RATAPC
  • APC1
  • adenomatous polyposis coli
  • adenomatous polyposis coli protein
  • adenomatosis polyposis coli
  • APC
  • apc
  • Smp_139190
  • Apc
Epitope
pSer2054
25
25
22
19
15
9
9
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
166
104
72
10
7
6
5
4
3
2
2
1
Host
Rabbit
150
37
15
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
7
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
138
75
61
57
46
26
26
22
15
4
2
2
1
1
1
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Catalog No. ABIN1531521
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1532537 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1533213 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN3183309 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2878980 IHC (p) ELISA WB Rabbit IgG AA 2794-2843 Log in to see Polyclonal
1 ABIN2888780 IHC (p) ELISA Rabbit IgG AA 2020-2069 Log in to see Polyclonal
1 ABIN1731843 IHC (p) ELISA WB Rabbit IgG AA 2794-2843 Log in to see Polyclonal
1 ABIN1585865 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN3182607 ELISA IHC WB Rabbit IgG pSer2054 Log in to see Polyclonal
1 ABIN3183308 ELISA IHC Rabbit IgG Ser142 Log in to see Polyclonal
1 ABIN1858037 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 2634-2843 Log in to see Polyclonal
1 ABIN559888 IF PLA ELISA WB Mouse IgG1 kappa AA 2744-2843, partial Log in to see 3E2
1 ABIN1827364 IF ELISA WB Mouse IgG1, kappa AA 2744-2843 Log in to see 3E2
1 ABIN2616762 ELISA WB Biotin Rabbit IgG AA 2634-2843 Log in to see Polyclonal
1 ABIN2616763 ELISA WB FITC Rabbit IgG AA 2634-2843 Log in to see Polyclonal
1 ABIN2883476 ELISA IHC (p) WB Rabbit IgG pSer2054 Log in to see Polyclonal
1 ABIN559887 ELISA WB Mouse AA 2744-2843, partial Log in to see Polyclonal
1 ABIN2616768 ELISA WB Rabbit IgG AA 1148-1176 Log in to see Polyclonal
1 ABIN2616757 ELISA WB Rabbit IgG AA 2634-2843 Log in to see Polyclonal
1 ABIN291141 IHC ELISA WB Goat IgG AA 2537-2843 Log in to see Polyclonal
1 ABIN5572678 ELISA IHC (p) WB Rabbit Log in to see Polyclonal

Similar anti-Adenomatous Polyposis Coli Antibodies

Application / Reactivity Human
ELISA 37 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 35 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 57 Antibodies
Immunoprecipitation (IP) 13 Antibodies
In Vivo Imaging (IVI) 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 102 Antibodies

General

Antigen Adenomatous Polyposis Coli (APC) Antibodies
Epitope pSer2054
(25), (25), (22), (19), (15), (9), (9), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(166), (104), (72), (10), (7), (6), (5), (4), (3), (2), (2), (1)
Host Rabbit
(150), (37), (15), (2), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(10), (10), (7), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(138), (75), (61), (57), (46), (26), (26), (22), (15), (4), (2), (2), (1), (1), (1)
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Product details

Target details Application Details Handling Images
Specificity APC (Phospho-Ser2054) Antibody detects endogenous levels of APC only when phosphorylated at Ser2054.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human APC around the phosphorylation site of Ser2054.
Isotype IgG

Target details

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Antigen
Alternative Name APC (APC Antibody Abstract)
Background Molecular weight of target protein: 311 kDa
Synonyms: Adenomatous polyposis coli protein, adenomatous polyposis of the colon gene, APC, APC protein, DP2.5, mAPC
Molecular Weight 311 kDa
Gene ID 324
OMIM 135290
UniProt P25054
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, Wnt, Transcription Factors, Coagulation
Pathways WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization

Application Details

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Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.158932 (NCBI Gene Symbol: APC)

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Adenomatous Polyposis Coli (APC) (pSer2054) antibody (ABIN1531521) Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30', usi...
Immunohistochemistry (IHC) image for anti-Adenomatous Polyposis Coli (APC) (pSer2054) antibody (ABIN1531521) Immunohistochemistry analysis of paraffin-embedded human brain, using APC (Phospho-Se...