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anti-Human Adenomatous Polyposis Coli antibody for ELISA

Recommended Adenomatous Polyposis Coli Antibody (supplied by: Log in to see )

Antigen
Adenomatous Polyposis Coli (APC) Antibodies
  • AI047805
  • APC1
  • AU020952
  • AW124434
  • BTPS2
  • CC1
  • dp2
  • DP2
  • DP2.5
  • dp2.5
  • DP3
  • dp3
  • fap
  • fpc
  • GS
  • mAPC
  • Min
  • PPP1R46
  • RATAPC
  • xapc
Epitope
pSer2054
23
22
17
15
13
10
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
114
73
42
6
4
2
2
1
1
Host
Rabbit
123
15
14
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
4
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
93
60
40
30
26
22
13
9
5
4
2
1
1
Supplier
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Catalog No. ABIN1531521
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012085857 ABIN1533213 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012085857 ABIN1532537 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012085857 ABIN2878980 IHC (p) ELISA WB Rabbit IgG AA 2794-2843 Log in to see Polyclonal
0.0012085857 ABIN2888780 IHC (p) ELISA Rabbit IgG AA 2020-2069 Log in to see Polyclonal
0.0012085857 ABIN2162634 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012085857 ABIN2162633 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012085857 ABIN1731843 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012085857 ABIN1585865 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012085857 ABIN291141 IHC ELISA WB Goat IgG AA 2537-2843 Log in to see Polyclonal
0.0012085857 ABIN3183308 ELISA IHC Rabbit IgG Ser142 Log in to see Polyclonal
0.0012085857 ABIN3183309 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012085857 ABIN3182607 ELISA IHC WB Rabbit IgG pSer2054 Log in to see Polyclonal
0.0012085857 ABIN1858037 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 2634-2843 Log in to see Polyclonal
0.0012085857 ABIN559888 IF PLA ELISA WB Mouse IgG1 kappa AA 2744-2843, partial Log in to see 3E2
0.0012085857 ABIN2616768 ELISA Rabbit IgG AA 1148-1176 Log in to see Polyclonal
0.0012085857 ABIN559887 ELISA WB Mouse AA 2744-2843, partial Log in to see Polyclonal
0.0012085857 ABIN1827364 IF ELISA WB Mouse IgG1, kappa Log in to see 3E2
0.0012085857 ABIN2883476 IHC ELISA WB Rabbit IgG pSer2054 Log in to see Polyclonal
0.0012085857 ABIN2616762 ELISA WB Biotin Rabbit IgG AA 2634-2843 Log in to see Polyclonal
0.0012085857 ABIN2616757 ELISA Rabbit IgG AA 2634-2843 Log in to see Polyclonal

Similar anti-Adenomatous Polyposis Coli Antibodies

Application / Reactivity Human
ELISA 34 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 61 Antibodies

General

Antigen Adenomatous Polyposis Coli (APC) Antibodies
Epitope pSer2054
(23), (22), (17), (15), (13), (10), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(114), (73), (42), (6), (4), (2), (2), (1), (1)
Host Rabbit
(123), (15), (14), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(7), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(93), (60), (40), (30), (26), (22), (13), (9), (5), (4), (2), (1), (1)
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Product details

Target details Application Details Handling Images
Specificity APC (Phospho-Ser2054) Antibody detects endogenous levels of APC only when phosphorylated at Ser2054.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human APC around the phosphorylation site of Ser2054.
Isotype IgG

Target details

Product details Application Details Handling Images back to top
Antigen
Alternative Name APC (APC Antibody Abstract)
Background Molecular weight of target protein: 311 kDa
Synonyms: Adenomatous polyposis coli protein, adenomatous polyposis of the colon gene, APC, APC protein, DP2.5, mAPC
Molecular Weight 311 kDa
Gene ID 324
OMIM 135290
UniProt P25054
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, Wnt, Transcription Factors, Coagulation
Pathways WNT Signaling

Application Details

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Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.158932 (NCBI Gene Symbol: APC)

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Adenomatous Polyposis Coli (APC) (pSer2054) antibody (ABIN1531521) Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30', usi...
Immunohistochemistry (IHC) image for anti-Adenomatous Polyposis Coli (APC) (pSer2054) antibody (ABIN1531521) Immunohistochemistry analysis of paraffin-embedded human brain, using APC (Phospho-Se...