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anti-Human Adenomatous Polyposis Coli antibody for Western Blotting

Recommended Adenomatous Polyposis Coli Antibody (supplied by: Log in to see )

Antigen
Adenomatous Polyposis Coli (APC) Antibodies
  • AI047805
  • APC1
  • AU020952
  • AW124434
  • BTPS2
  • CC1
  • dp2
  • DP2
  • DP2.5
  • dp2.5
  • DP3
  • dp3
  • fap
  • fpc
  • GS
  • mAPC
  • Min
  • PPP1R46
  • RATAPC
  • xapc
Epitope
pSer2054
23
22
21
17
15
13
10
4
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
152
90
58
6
4
2
2
1
1
Host
Rabbit
142
33
15
2
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
6
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
129
60
60
49
44
27
23
22
13
4
2
1
1
Supplier
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Catalog No. ABIN1531521
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN1533213 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2704484 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.13476625 ABIN2705480 WB Rabbit pSer2054 Log in to see Polyclonal
0.13476625 ABIN2162634 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN1858037 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 2634-2843 Log in to see Polyclonal
0.13476625 ABIN3183309 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.13476625 ABIN513388 PLA WB Rabbit AA 1-2843, full length Log in to see Polyclonal
0.0011760078 ABIN271993 IHC (p) WB Rabbit Log in to see Polyclonal
0.0011760078 ABIN265313 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0011760078 ABIN393899 IF WB Mouse IgG1 kappa AA 2744-2845 Log in to see 3E2 5
0.0011760078 ABIN373037 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0011760078 ABIN152091 IHC (fro) IP IHC WB Mouse IgG1 N-Term Log in to see ALi 12-28 2
0.0011760078 ABIN2878980 IHC (p) ELISA WB Rabbit IgG AA 2794-2843 Log in to see Polyclonal
0.0011760078 ABIN1803997 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.0011760078 ABIN2470732 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011760078 ABIN224804 IHC (fro) WB Mouse IgG1 AA 1-433 Log in to see
0.0011760078 ABIN1731843 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011760078 ABIN1585865 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011760078 ABIN2891311 IF IHC WB Rabbit Gln2829 Log in to see Polyclonal
0.0011760078 ABIN2852374 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-Adenomatous Polyposis Coli antibody for Western Blotting

Similar anti-Adenomatous Polyposis Coli Antibodies

Application / Reactivity Human
ELISA 34 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 35 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 97 Antibodies

General

Antigen Adenomatous Polyposis Coli (APC) Antibodies
Epitope pSer2054
(23), (22), (21), (17), (15), (13), (10), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(152), (90), (58), (6), (4), (2), (2), (1), (1)
Host Rabbit
(142), (33), (15), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(9), (8), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(129), (60), (60), (49), (44), (27), (23), (22), (13), (4), (2), (1), (1)
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Product details

Target details Application Details Handling Images
Specificity APC (Phospho-Ser2054) Antibody detects endogenous levels of APC only when phosphorylated at Ser2054.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human APC around the phosphorylation site of Ser2054.
Isotype IgG

Target details

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Antigen
Alternative Name APC (APC Antibody Abstract)
Background Molecular weight of target protein: 311 kDa
Synonyms: Adenomatous polyposis coli protein, adenomatous polyposis of the colon gene, APC, APC protein, DP2.5, mAPC
Molecular Weight 311 kDa
Gene ID 324
OMIM 135290
UniProt P25054
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, Wnt, Transcription Factors, Coagulation
Pathways WNT Signaling

Application Details

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Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.158932 (NCBI Gene Symbol: APC)

Restrictions For Research Use only

Handling

Product details Target details Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product details Target details Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Adenomatous Polyposis Coli (APC) (pSer2054) antibody (ABIN1531521) Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30', usi...
Immunohistochemistry (IHC) image for anti-Adenomatous Polyposis Coli (APC) (pSer2054) antibody (ABIN1531521) Immunohistochemistry analysis of paraffin-embedded human brain, using APC (Phospho-Se...