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anti-Xenopus laevis DKK1 antibody for Western Blotting

Recommended DKK1 Antibody (supplied by: Log in to see )

Antigen
Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) Antibodies
  • AI414495
  • CT34
  • dickkopf-1
  • Dickkopf1
  • dkk
  • DKK-1
  • dkk-1
  • dkk1
  • dkk1-A
  • mdkk-1
  • mSgy
  • SGY
  • SGY-1
  • SGY1
  • SK
  • Soggy
  • Xdkk-1
  • xdkk1
Epitope
C-Term
34
31
16
13
9
8
6
6
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
190
42
40
18
5
3
3
2
2
1
1
1
1
Host
Rabbit
141
39
20
2
1
Clonality
Polyclonal
Conjugate
This DKK1 antibody is un-conjugated
9
7
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
144
91
34
30
11
5
4
4
3
3
1
1
1
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Catalog No. ABIN2773909
$ 229.00
Plus shipping costs $45.00

Top referenced anti-DKK1 antibody for Western Blotting

Similar anti-DKK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine) Pig (Porcine) Rabbit Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 138 Antibodies 29 Antibodies 25 Antibodies 1 Antibodies 4 Antibodies 9 Antibodies 4 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies 6 Antibodies 4 Antibodies 5 Antibodies 1 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 28 Antibodies

General

Antigen Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) Antibodies
Epitope C-Term
(34), (31), (16), (13), (9), (8), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(190), (42), (40), (18), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(141), (39), (20), (2), (1)
Clonality
Polyclonal
Conjugate This DKK1 antibody is un-conjugated
(9), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(144), (91), (34), (30), (11), (5), (4), (4), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-DKK1 Antibody

Target Details DKK1 Application Details Handling References for anti-DKK1 Antibody (ABIN2773909) Images
Sequence CARHFWSKIC KPVLKEGQVC TKHRRKGSHG LEIFQRCYCG EGLSCRIQKD
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Dog : 100 %, Goat : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Mouse : 90 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against DKK1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human DKK1

Target Details DKK1

Product Details anti-DKK1 Antibody Application Details Handling References for anti-DKK1 Antibody (ABIN2773909) Images back to top
Antigen
Alternative Name DKK1 (DKK1 Antibody Abstract)
Background DKK1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Molecular Weight 26 kDa
Gene ID 22943
NCBI Accession NM_012242, NP_036374
UniProt O94907
Research Area Embryogenesis, Wnt
Pathways WNT Signaling

Application Details

Product Details anti-DKK1 Antibody Target Details DKK1 Handling References for anti-DKK1 Antibody (ABIN2773909) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 266 AA

Restrictions For Research Use only

Handling

Product Details anti-DKK1 Antibody Target Details DKK1 Application Details References for anti-DKK1 Antibody (ABIN2773909) Images back to top
Format Liquid
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DKK1 Antibody (ABIN2773909)

Product Details anti-DKK1 Antibody Target Details DKK1 Application Details Handling Images back to top
Background publications

Ai, Holmen, Van Hul, Williams, Warman: "Reduced affinity to and inhibition by DKK1 form a common mechanism by which high bone mass-associated missense mutations in LRP5 affect canonical Wnt signaling." in: Molecular and cellular biology, Vol. 25, Issue 12, pp. 4946-55, 2005

Images

Product Details anti-DKK1 Antibody Target Details DKK1 Application Details Handling References for anti-DKK1 Antibody (ABIN2773909) back to top
Supplier Images
Western Blotting (WB) image for anti-DKK1 antibody (Dickkopf Homolog 1 (Xenopus Laevis)) (C-Term) (ABIN2773909) WB Suggested Anti-DKK1 Antibody Titration: 2.5ug/ml
Positive Control: