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anti-Human FZD4 antibody for Immunocytochemistry

Recommended FZD4 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 4 (FZD4) Antibodies
  • CG4626
  • DFz4
  • Dfz4
  • Dm Fz4
  • Dmel\\CG4626
  • Fz4
  • anon-WO0170980.10
  • anon-WO0170980.11
  • fz1
  • zg01
  • CD344
  • EVR1
  • FEVR
  • FZD4S
  • Fz-4
  • FzE4
  • GPCR
  • hFz4
  • frizzled4
  • fz4
  • FZ-4
  • frizzled 4
  • frizzled homolog 4
  • frizzled-4
  • frizzled family receptor 4
  • frizzled homolog 4 (Drosophila)
  • fz4
  • fzd4
  • Tsp_10376
  • FZD4
  • Fzd4
Epitope
AA 1-180
33
16
15
13
11
8
4
3
3
3
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
117
62
45
4
3
3
2
2
2
2
2
2
1
1
1
Host
Rat
78
26
14
8
Clonality
Monoclonal
Conjugate
This FZD4 antibody is conjugated to Biotin
7
6
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
73
55
17
13
13
12
11
5
2
1
1
Supplier
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Catalog No. ABIN4898976
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4900303 FACS ICC WB Rat IgG2a AA 37-180 Log in to see 145901 2
1 ABIN4900304 FACS ICC WB Rat IgG2a AA 37-180 Log in to see 145901 2
1 ABIN1852878 ICC IF ELISA Rabbit Internal Region Log in to see Polyclonal

Top referenced anti-FZD4 antibody for Immunocytochemistry

Similar anti-FZD4 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 18 Antibodies 52 Antibodies
Flow Cytometry (FACS) 15 Antibodies 15 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 4 Antibodies
Immunofluorescence (IF) 9 Antibodies 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 13 Antibodies
Western Blotting (WB) 30 Antibodies 65 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Frizzled Family Receptor 4 (FZD4) Antibodies
Epitope AA 1-180
(33), (16), (15), (13), (11), (8), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(117), (62), (45), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rat
(78), (26), (14), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FZD4 antibody is conjugated to Biotin
(7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(73), (55), (17), (13), (13), (12), (11), (5), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-FZD4 Antibody

Target Details FZD4 Application Details Handling References for anti-FZD4 Antibody (ABIN4898976) Images
Specificity Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.15 EU per 1 g of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4, Met1-Glu180, Accession # Q61088
Clone 145901
Isotype IgG2a

Target Details FZD4

Product Details anti-FZD4 Antibody Application Details Handling References for anti-FZD4 Antibody (ABIN4898976) Images back to top
Antigen
Alternative Name Frizzled-4 (FZD4 Antibody Abstract)
Gene ID 8322
UniProt Q61088
Pathways WNT Signaling

Application Details

Product Details anti-FZD4 Antibody Target Details FZD4 Handling References for anti-FZD4 Antibody (ABIN4898976) Images back to top
Application Notes Western Blot 1 μg/mL, Flow Cytometry 0.25 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details References for anti-FZD4 Antibody (ABIN4898976) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with BSA as a carrier protein.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-FZD4 Antibody (ABIN4898976)

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Handling Images back to top
Product cited in:

Despeaux, Chicanne, Rouer, De Toni-Costes, Bertrand, Mansat-De Mas, Vergnolle, Eaves, Payrastre, Girault, Racaud-Sultan: "Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered Wnt signaling." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 8, pp. 1597-610, 2012 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).