|Application / Reactivity||Human||Mouse (Murine)||Cow (Bovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||1 Antibodies|
|Western Blotting (WB)||95 Antibodies||7 Antibodies||40 Antibodies|
|Neutralization (Neut)||3 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies||5 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies||32 Antibodies||18 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Antigen||Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine) Alternatives|
|Conjugate||This IGFBP4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-IGFBP4 AntibodyTarget Details IGFBP4 Application Details Handling References for anti-IGFBP4 Antibody (ABIN374952) Images
|Specificity||This antibody reacts to IGFBP4.|
Species reactivity (expected):Mouse, Bovine.
Species reactivity (tested):Human.
|Immunogen||Peptide with sequence KGELDCHQLADSFRE, from the C Terminus of the protein sequence|
Target Details IGFBP4Product Details anti-IGFBP4 Antibody Application Details Handling References for anti-IGFBP4 Antibody (ABIN374952) Images back to top
|Alternative Name||IGFBP4 (IGFBP4 Antibody Abstract)|
|Background||IGFBP4 (Insulin like growth factor binding protein 4) is produced from a DNA sequence encoding the human IGFBP4 protein. Mature human IGFBP4 has a calculated molecular mass of approximately 26 kDa. Due to glycosylation, the recombinant protein migrates as a 32 kDa and 25 kDa protein under reducing and non reducing conditions, respectively. Human IGFBP4 has a potential N-linked glycosylation site and shares approximately 90 % amino acid sequence identity with both mouse and rat IGFBP4. IGFBP4 is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). IGFBP4 functions as an inhibitor of IGF action and its main function may be to protect cells from overstimulation by IGFs or to allow activation of alternate transmembrane signaling pathways that are inhibited by IGF exposure. IGFBP4 is expressed in multiple tissues including adrenal, testis, spleen, heart, lung, kidney, liver, stomach, hypothalamus, and brain cortex.Synonyms: IBP-4, IBP4, IGF-binding protein 4, IGFBP-4, Insulin-like growth factor-binding protein 4|
|Research Area||Signaling, Extracellular Matrix, Angiogenesis|
|Pathways||WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process|
Application DetailsProduct Details anti-IGFBP4 Antibody Target Details IGFBP4 Handling References for anti-IGFBP4 Antibody (ABIN374952) Images back to top
Peptide ELISA: 1/8000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IGFBP4 Antibody Target Details IGFBP4 Application Details References for anti-IGFBP4 Antibody (ABIN374952) Images back to top
|Buffer||Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-IGFBP4 Antibody (ABIN374952)Product Details anti-IGFBP4 Antibody Target Details IGFBP4 Application Details Handling Images back to top
Durai, Davies, Yang, Yang, Seifalian, Goldspink, Winslet: "Biology of insulin-like growth factor binding protein-4 and its role in cancer (review)." in: International journal of oncology, Vol. 28, Issue 6, pp. 1317-25, 2006
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