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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)||Cow (Bovine)||Dog (Canine)||Goat||Horse (Equine)||Sheep (Ovine)||Rabbit||Bat|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||95 Antibodies||7 Antibodies||40 Antibodies||3 Antibodies||20 Antibodies||2 Antibodies||3 Antibodies||3 Antibodies||3 Antibodies||1 Antibodies|
|Neutralization (Neut)||3 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||1 Antibodies||2 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Antigen||Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) Antibodies|
|Epitope||AA 180-229 Alternatives|
|Reactivity||Bat, Cow (Bovine), Dog (Canine), Goat, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This IGFBP4 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-IGFBP4 AntibodyTarget Details IGFBP4 Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Sheep, Dog, Bovine, Horse (100%) Pig, Guinea pig, Chicken (92%).|
Synthetic peptide located between aa180-229 of human IGFBP4 (P22692, NP_001543). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse (100%), Marmoset, Hamster, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (92%), Opossum (85%).
Type of Immunogen: Synthetic peptide
Target Details IGFBP4Product Details anti-IGFBP4 Antibody Application Details Handling Images back to top
|Alternative Name||IGFBP4 / IGFBP-4 (IGFBP4 Antibody Abstract)|
Name/Gene ID: IGFBP4
Synonyms: IGFBP4, BP-4, HT29-IGFBP, IGF-binding protein 4, IBP-4, IGFBP-4, IBP4
|Pathways||WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process|
Application DetailsProduct Details anti-IGFBP4 Antibody Target Details IGFBP4 Handling Images back to top
Approved: IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
|Restrictions||For Research Use only|
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|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
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