anti-Rat (Rattus) IGFBP4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended IGFBP4 Antibody (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) Antibodies
  • IGFBP4
  • igfbp4
  • BP-4
  • HT29-IGFBP
  • IBP4
  • IGFBP-4
  • AI875747
  • Deb2
  • IGF-BP4
  • IBP-4
  • insulin-like growth factor binding protein 4
  • IGFBP4
  • igfbp4
  • LOC100194498
  • LOC100225608
  • LOC100271886
  • Igfbp4
  • LOC100337926
Epitope
AA 180-229
56
34
14
12
11
10
8
7
5
5
2
2
2
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Goat, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
219
139
19
6
5
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
138
84
17
Clonality
Polyclonal
Conjugate
This IGFBP4 antibody is un-conjugated
16
15
11
10
6
6
6
6
6
6
6
6
6
6
6
5
5
5
5
5
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
190
124
41
10
9
7
5
3
3
3
3
3
2
2
2
2
1
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Catalog No. ABIN490041
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1859306 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 22-254 Log in to see

General

Antigen Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) Antibodies
Epitope AA 180-229
(56), (34), (14), (12), (11), (10), (8), (7), (5), (5), (2), (2), (2), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Goat, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(219), (139), (19), (6), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(138), (84), (17)
Clonality
Polyclonal
Conjugate This IGFBP4 antibody is un-conjugated
(16), (15), (11), (10), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(190), (124), (41), (10), (9), (7), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
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Product Details anti-IGFBP4 Antibody

Target Details IGFBP4 Application Details Handling Images
Brand IHC-plus™
Specificity Human IGFBP4
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Sheep, Dog, Bovine, Horse (100%) Pig, Guinea pig, Chicken (92%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa180-229 of human IGFBP4 (P22692, NP_001543). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse (100%), Marmoset, Hamster, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details IGFBP4

Product Details anti-IGFBP4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IGFBP4 / IGFBP-4 (IGFBP4 Antibody Abstract)
Background Name/Gene ID: IGFBP4

Synonyms: IGFBP4, BP-4, HT29-IGFBP, IGF-binding protein 4, IBP-4, IGFBP-4, IBP4
Gene ID 3487
NCBI Accession NP_001543
UniProt P22692
Pathways WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-IGFBP4 Antibody Target Details IGFBP4 Handling Images back to top
Application Notes Approved: IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Restrictions For Research Use only

Handling

Product Details anti-IGFBP4 Antibody Target Details IGFBP4 Application Details Images back to top
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-IGFBP4 Antibody Target Details IGFBP4 Application Details Handling back to top
Supplier Images
 image for anti-Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) (AA 180-229) antibody (ABIN490041) Colon, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
 image for anti-Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) (AA 180-229) antibody (ABIN490041) Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
 image for anti-Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) (AA 180-229) antibody (ABIN490041) Prostate, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)