Mouse (Murine) IGFBP5 ELISA Kit

Recommended IGFBP5 Product (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kits
  • igfbp5
  • ibp5
  • IBP-5
  • IGFBP-5
  • xIGFBP-5
  • IGFBP5
  • IBP5
  • AI256729
  • AW208790
  • IGFBP-5P
  • IGF-BP5
  • insulin-like growth factor binding protein 5a
  • insulin-like growth factor binding protein 5
  • insulin-like growth factor-binding protein 5
  • igfbp5a
  • igfbp5
  • IGFBP5
  • LOC100194500
  • Igfbp5
  • LOC100348897
  • IGFBP-5
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
18
15
15
8
4
2
2
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
5-3000 pg/mL
Minimum Detection Limit
5 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN625131
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.497856 ABIN1889356 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 156-10.000 pg/mL 156 pg/mL Log in to see 2
12.497856 ABIN2703127 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 25-7.000 pg/mL 25 pg/mL Log in to see
12.497856 ABIN2708528 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN841741 Sandwich ELISA Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN4993103 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-3000 pg/mL 4.1 pg/mL Log in to see
1 ABIN628015 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
1 ABIN1884553 Sandwich ELISA Plasma, Serum, Biological Fluids 125-8000 pg/mL 125 pg/mL Log in to see
1 ABIN5523443 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN4885924 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Log in to see
1 ABIN4885755 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Log in to see
1 ABIN5596549 Sandwich ELISA Log in to see
1 ABIN5596294 Sandwich ELISA Log in to see
1 ABIN2948557 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN1138187 Log in to see
1 ABIN5069122 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

General

Antigen Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(18), (15), (15), (8), (4), (2), (2), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 5-3000 pg/mL
Minimum Detection Limit 5 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details IGFBP5 ELISA Kit

Target details Application Details Handling References for IGFBP5 Kit (ABIN625131) Images
Purpose Mouse IGFBP-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Serum, Plasma, Cell Culture Supernatant
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity < 5 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IGFBP5 ELISA Kit Application Details Handling References for IGFBP5 Kit (ABIN625131) Images back to top
Antigen
Alternative Name IGFBP-5 (IGFBP5 ELISA Kit Abstract)
Background The Mouse IGFBP-5 (Insulin-like growth factor binding protein 5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP-5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for IGFBP-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 16011
UniProt Q07079
Research Area Diabetes, Metabolism, Growth Factors
Pathways WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding

Application Details

Product Details IGFBP5 ELISA Kit Target details Handling References for IGFBP5 Kit (ABIN625131) Images back to top
Application Notes Recommended Dilution for serum and plasma samples100 - 1,000 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent C (Item L) should be used for dilution of serum/plasma/culture supernatants. Suggested dilution for normal serum/plasma: 100-1000 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B (Item E) should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL Assay Diluent C (Item L) into Item C vial to prepare a 100 ng/mL standard solution. Dissolve the powder thoroughly by a gentle mix. Add 30 µL IGFBP-5 standard from the vial of Item C, into a tube with 970 µL Assay Diluent C to prepare a 3,000 pg/mL standard solution. Pipette 400myl Assay Diluent C into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent C serves as the zero standard (0 pg/mL). 200 µL 30 µL standard + 970 µL 200myl 200 µL 200 µL 200 µL 200 µL 3,000 1,000 333.3 111.1 37.04 12.35 4.12 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B (Item E) into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent B (Item E). For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 200-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Mouse IGFBP-5 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent C
Sensitivity: The minimum detectable dose of IGFBP-5 is typically less than 5 pg/mL.
Recovery: Recovery was determined by spiking various levels of IGFBP-5 into normal Mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 115.3 86-130 Plasma 95.11 83-102 Cell culture media 91.26 80-101
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 73.54 98.44 97.32 Range ( %) 68-86 87-106 85-104 1:4 Average % of Expected 71.58 130.5 137.6 Range ( %) 65-84 118-140 123-145
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details IGFBP5 ELISA Kit Target details Application Details References for IGFBP5 Kit (ABIN625131) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for IGFBP5 Kit (ABIN625131)

Product Details IGFBP5 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Talbot, Sparks, Powell, Kahl, Caperna: "Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells." in: In vitro cellular & developmental biology. Animal, Vol. 48, Issue 1, pp. 1-11, 2012

Images

Product Details IGFBP5 ELISA Kit Target details Application Details Handling References for IGFBP5 Kit (ABIN625131) back to top
Supplier Images
ELISA image for Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit (ABIN625131) Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit