You are viewing an incomplete version of our website. Please click to reload the website as full version.

Mouse (Murine) IGFBP5 ELISA Kit

Recommended IGFBP5 Product (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kits
  • AI256729
  • AW208790
  • IBP-5
  • IBP5
  • ibp5
  • IGF-BP5
  • IGFBP-5
  • IGFBP-5P
  • IGFBP5
  • igfbp5
  • xIGFBP-5
Epitope
AA 20-271
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
14
12
9
7
4
3
3
2
2
2
2
2
Methode Type
Sandwich ELISA
Detection Range
156-10.000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1889356
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN2708528 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.0012092679 ABIN841741 Sandwich ELISA Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN628015 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 5-100 ng/mL 5 ng/mL Log in to see
0.0012092679 ABIN1884553 Sandwich ELISA 125-8000 pg/mL 125 pg/mL Log in to see
0.0012092679 ABIN625131 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see
0.0012092679 ABIN2703127 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 25-7.000 pg/mL 25 pg/mL Log in to see
0.0012092679 ABIN1138187 Log in to see
0.0012092679 ABIN2088071 Sandwich ELISA Log in to see
0.0012092679 ABIN2948557 0.156-10 ng/mL 0.156 ng/mL Log in to see

Top referenced IGFBP5 ELISA Kit for ELISA

Similar IGFBP5 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 10 ELISA Kits

General

Antigen Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kits
Epitope AA 20-271
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(14), (12), (9), (7), (4), (3), (3), (2), (2), (2), (2), (2)
Methode Type Sandwich ELISA
Detection Range 156-10.000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details IGFBP5 ELISA Kit

Target details Application Details Handling References for IGFBP5 Kit (ABIN1889356) Images
Purpose For quantitative detection of mouse IGFBP5 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse IGFBP5
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse IGFBP5 antibod - 1
  • Lyophilized recombinant mouse IGFBP5 standar - 10ng/tubex2
  • Biotinylated anti- mouse IGFBP5 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: L20-E271

Target details

Product Details IGFBP5 ELISA Kit Application Details Handling References for IGFBP5 Kit (ABIN1889356) Images back to top
Antigen
Alternative Name IGFBP5 (IGFBP5 ELISA Kit Abstract)
Background Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.
Gene ID 16011
UniProt Q07079
Research Area Signaling, Diabetes, Metabolism, Growth Factors
Pathways WNT Signaling

Application Details

Product Details IGFBP5 ELISA Kit Target details Handling References for IGFBP5 Kit (ABIN1889356) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP5 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IGFBP5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP5 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.1, Standard deviation: 0.045, CV(%): 4.1
  • Sample 2: n=16, Mean(ng/ml): 3.4, Standard deviation: 0.167, CV(%): 4.9
  • Sample 3: n=16, Mean(ng/ml): 6.2, Standard deviation: 0.341, CV(%): 5.5,
  • Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.084, CV(%): 5.6
  • Sample 2: n=24, Mean(ng/ml): 3.8, Standard deviation: 0.247, CV(%): 6.5
  • Sample 3: n=24, Mean(ng/ml): 6.7, Standard deviation: 0.476, CV(%): 7.1
Restrictions For Research Use only

Handling

Product Details IGFBP5 ELISA Kit Target details Application Details References for IGFBP5 Kit (ABIN1889356) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IGFBP5 Kit (ABIN1889356)

Product Details IGFBP5 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Butt, Dickson, McDougall, Baxter: "Insulin-like growth factor-binding protein-5 inhibits the growth of human breast cancer cells in vitro and in vivo." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29676-85, 2003

Allander, Larsson, Ehrenborg, Suwanichkul, Weber, Morris, Bajalica, Kiefer, Luthman, Powell: "Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5." in: The Journal of biological chemistry, Vol. 269, Issue 14, pp. 10891-8, 1994

Images

Product Details IGFBP5 ELISA Kit Target details Application Details Handling References for IGFBP5 Kit (ABIN1889356) back to top
Supplier Images
ELISA image for IGFBP5 ELISA Kit (Insulin-Like Growth Factor Binding Protein 5) (ABIN1889356) Mouse IGFBP5 PicoKine ELISA Kit standard curve