anti-Dog (Canine) LEF1 antibody for ELISA

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Epitope
AA 257-270
15
13
13
9
8
6
6
5
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish
122
39
31
10
7
6
4
4
4
4
3
3
2
1
1
Host
Goat
54
49
15
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
ELISA, Western Blotting (WB)
110
65
21
15
8
7
4
3
2
2
2
1
1
1
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Catalog No. ABIN297259
$ 595.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2460459 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2563660 ELISA WB Goat IgG Internal Region Log in to see Polyclonal

Similar anti-LEF1 Antibodies

Application / Reactivity Human Chicken Cow (Bovine) Dog (Canine) Mouse (Murine) Pig (Porcine) Rat (Rattus) Zebrafish Horse (Equine) Bat Monkey Hamster
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Western Blotting (WB) 110 Antibodies 3 Antibodies 7 Antibodies 11 Antibodies 34 Antibodies 5 Antibodies 26 Antibodies 4 Antibodies 5 Antibodies 2 Antibodies 4 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunoprecipitation (IP) 3 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope AA 257-270
(15), (13), (13), (9), (8), (6), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish
(122), (39), (31), (10), (7), (6), (4), (4), (4), (4), (3), (3), (2), (1), (1)
Host Goat
(54), (49), (15), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application ELISA, Western Blotting (WB)
(110), (65), (21), (15), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling Images
Specificity Human LEF1.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%) Xenopus (93%) Pufferfish (86%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide C-QHEQRKEQEPKRPH from an internal region of human LEF1 (NP_057353.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%), Xenopus (93%), Pufferfish (86%).

Type of Immunogen: Synthetic peptide

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA
Gene ID 51176
NCBI Accession NP_057353
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers, Wnt Signaling
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Application Notes Approved: ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). Recommended concentration: 1-3 μg/mL.

Not recommended for: IHC-P
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. Minimize freezing and thawing.

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling back to top
Supplier Images
 image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 257-270) (ABIN297259) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 257-270) antibody