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anti-Human LEF1 antibody for Biochemical Assay

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Reactivity
Human
117
33
25
7
4
4
3
2
1
1
1
1
1
Host
Mouse
53
44
16
5
Clonality
Monoclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Biochemical Assay (BCA)
108
62
19
11
5
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2666400
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088537845 ABIN2666401 BCA Mouse IgG1 kappa Log in to see 15H5A18 6

Top referenced anti-LEF1 antibody for Biochemical Assay

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General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Reactivity Human
(117), (33), (25), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(53), (44), (16), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Biochemical Assay (BCA)
(108), (62), (19), (11), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2666400) Images
Clone 15H5A18
Isotype IgG1 kappa

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN2666400) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR- α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN2666400) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN2666400) Images back to top
Concentration 0.5 mg/mL
Buffer phosphate-buffered solution, pH 7.2, containing 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Upon receipt, store undiluted between 2°C and 8°C.

References for anti-LEF1 Antibody (ABIN2666400)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Background publications

Mallory, Jackson, Weber, Chi, Heyd, Lynch: "Signal- and development-dependent alternative splicing of LEF1 in T cells is controlled by CELF2." in: Molecular and cellular biology, Vol. 31, Issue 11, pp. 2184-95, 2011

Petropoulos, Arseni, Schessl, Stadler, Rawat, Deshpande, Heilmeier, Hiddemann, Quintanilla-Martinez, Bohlander, Feuring-Buske, Buske: "A novel role for Lef-1, a central transcription mediator of Wnt signaling, in leukemogenesis." in: The Journal of experimental medicine, Vol. 205, Issue 3, pp. 515-22, 2008

Nguyen, Rosner, Milovanovic, Hope, Planutis, Saha, Chaiwun, Lin, Imam, Marsh, Holcombe: "Wnt pathway component LEF1 mediates tumor cell invasion and is expressed in human and murine breast cancers lacking ErbB2 (her-2/neu) overexpression." in: International journal of oncology, Vol. 27, Issue 4, pp. 949-56, 2005

Hovanes, Li, Munguia, Truong, Milovanovic, Lawrence Marsh, Holcombe, Waterman: "Beta-catenin-sensitive isoforms of lymphoid enhancer factor-1 are selectively expressed in colon cancer." in: Nature genetics, Vol. 28, Issue 1, pp. 53-7, 2001

Bruhn, Munnerlyn, Grosschedl: "ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCRalpha enhancer function." in: Genes & development, Vol. 11, Issue 5, pp. 640-53, 1997

Waterman, Fischer, Jones: "A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer." in: Genes & development, Vol. 5, Issue 4, pp. 656-69, 1991

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2666400) back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (ABIN2666400) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody