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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||21 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||4 Antibodies|
|Immunohistochemistry (IHC)||13 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Western Blotting (WB)||110 Antibodies|
|Antigen||Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies|
|Conjugate||This LEF1 antibody is un-conjugated Alternatives|
Biochemical Assay (BCA)
|6 references available|
|Supplier||Log in to see|
Product Details anti-LEF1 AntibodyTarget Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2666400) Images
Target Details LEF1Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN2666400) Images back to top
|Alternative Name||LEF1 (LEF1 Antibody Abstract)|
|Background||Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR- α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.|
|Research Area||Transcription Factors, Wnt, Lineage Markers|
Application DetailsProduct Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN2666400) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN2666400) Images back to top
|Buffer||phosphate-buffered solution, pH 7.2, containing 0.05 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Upon receipt, store undiluted between 2°C and 8°C.|
References for anti-LEF1 Antibody (ABIN2666400)Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Mallory, Jackson, Weber et al.: "Signal- and development-dependent alternative splicing of LEF1 in T cells is controlled by CELF2." in: Molecular and cellular biology, Vol. 31, Issue 11, pp. 2184-95, 2011 (PubMed).
Petropoulos, Arseni, Schessl et al.: "A novel role for Lef-1, a central transcription mediator of Wnt signaling, in leukemogenesis." in: The Journal of experimental medicine, Vol. 205, Issue 3, pp. 515-22, 2008 (PubMed).
Nguyen, Rosner, Milovanovic et al.: "Wnt pathway component LEF1 mediates tumor cell invasion and is expressed in human and murine breast cancers lacking ErbB2 (her-2/neu) overexpression." in: International journal of oncology, Vol. 27, Issue 4, pp. 949-56, 2005 (PubMed).
Hovanes, Li, Munguia et al.: "Beta-catenin-sensitive isoforms of lymphoid enhancer factor-1 are selectively expressed in colon cancer." in: Nature genetics, Vol. 28, Issue 1, pp. 53-7, 2001 (PubMed).
Bruhn, Munnerlyn, Grosschedl: "ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCRalpha enhancer function." in: Genes & development, Vol. 11, Issue 5, pp. 640-53, 1997 (PubMed).
Waterman, Fischer, Jones: "A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer." in: Genes & development, Vol. 5, Issue 4, pp. 656-69, 1991 (PubMed).
ImagesProduct Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2666400) back to top