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anti-Human LEF1 antibody for Western Blotting

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Epitope
AA 10-37, N-Term
20
16
16
6
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
117
33
25
7
4
4
3
2
1
1
1
1
1
Host
Rabbit
52
45
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
107
62
18
11
5
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN655987
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.11933539 ABIN953158 EIA WB Rabbit Ig N-Term, AA 17-45 Log in to see Polyclonal 3
0.11933539 ABIN2779620 IHC WB Rabbit C-Term Log in to see Polyclonal 1
0.11933539 ABIN2792635 WB Rabbit N-Term Log in to see Polyclonal 1
0.11933539 ABIN1882259 WB Mouse IgG1 AA 10-37 Log in to see 661CT8-3-5 3
0.11933539 ABIN264381 IF WB Mouse IgG1 HMG Binding Domain Log in to see REMB6 1
0.11933539 ABIN2779621 WB Rabbit Middle Region Log in to see Polyclonal
0.11933539 ABIN735248 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
0.11933539 ABIN565556 RNAi ELISA WB Mouse IgG1 kappa AA 1-399, full length Log in to see 3H5 2
0.11933539 ABIN126828 EIA WB Mouse IgG1 Log in to see 2D12
0.11933539 ABIN264397 WB Mouse IgG1 Alternate Exon Log in to see 1C3
0.11933539 ABIN1531685 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
0.11933539 ABIN2460459 IHC ELISA WB Rabbit Log in to see Polyclonal
0.11933539 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal
0.11933539 ABIN2465242 ELISA WB Goat Internal Region Log in to see Polyclonal
0.11933539 ABIN2460133 ELISA WB Rabbit Log in to see Polyclonal
0.11933539 ABIN188546 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.11933539 ABIN395469 IF WB Mouse IgG1 kappa AA 14-124 Log in to see 2C9
0.11933539 ABIN396506 IF WB Mouse IgG1 kappa AA 14-124 Log in to see 5A3
0.11933539 ABIN2491171 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
0.11933539 ABIN2489190 WB Mouse IgG1 Log in to see 661CT8-3-5

Top referenced anti-LEF1 antibody for Western Blotting

Similar anti-LEF1 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 63 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
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Western Blotting (WB) 109 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope AA 10-37, N-Term
(20), (16), (16), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(117), (33), (25), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(52), (45), (16), (5)
Clonality (Clone)
Polyclonal   ( )
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Western Blotting (WB)
(107), (62), (18), (11), (5), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN655987) Images
Specificity This LEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human LEF1.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This LEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-37 AA from the N-terminal region of human LEF1.
Clone RB31989
Isotype Ig

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN655987) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Synonyms: LEF1,Lymphoid enhancer-binding factor 1,
Molecular Weight 44201 DA
Gene ID 51176
NCBI Accession NP_057353
UniProt Q9UJU2
Research Area Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Cell Structure
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN655987) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN655987) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-LEF1 Antibody (ABIN655987)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Product cited in:

Yao, Li, Liang, Lu, Zhu, Liu, Zhang, Yuan, Wang: "Methylprednisolone suppresses the Wnt signaling pathway in chronic lymphocytic leukemia cell line MEC-1 regulated by LEF-1 expression." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7921-8, 2015

Background publications

Gutierrez, Tschumper, Wu, Shanafelt, Eckel-Passow, Huddleston, Slager, Kay, Jelinek: "LEF-1 is a prosurvival factor in chronic lymphocytic leukemia and is expressed in the preleukemic state of monoclonal B-cell lymphocytosis." in: Blood, Vol. 116, Issue 16, pp. 2975-83, 2010

Kalsi, Kuo, Aliev, Alexander, McMichael, Patterson, Walsh, Zhao, Schuckit, Nurnberger, Edenberg, Kramer, Hesselbrock, Tischfield, Vladimirov, Prescott, Dick, Kendler, Riley: "A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts." in: Human molecular genetics, Vol. 19, Issue 12, pp. 2497-506, 2010

Chen, Zhang, Pincus, Wu, Ricks, Liu, Sun, Maclaren, Lan: "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 5047-54, 2010

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN655987) back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 10-37) (ABIN655987) LEF1 Antibody (N-term) (ABIN655987) western blot analysis in MDA-MB435 cell line lysa...